Donkey Anti-Goat IgG/Bio
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
20ul | CAB.29216-20ul | €100.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.29216-50ul | €500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.29216-100ul | €27.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul(PBS Only) | CAB.29216-100ul(PBS Only) | €500.00 |
| Catalog No | CAB.29216 |
|---|---|
| Product Name | Donkey Anti-Goat IgG/Bio |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | IF;IHC-F;IHC-P;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Goat IgG/Bio antibody is a versatile reagent applicable in various immunoassays.
For optimal performance, handle the antibody with care and follow the recommended dilutions.
Reagents or buffers: Tris-buffered saline with Tween 20 (TBST), non-fat dry milk.
Sample preparation: Resolve protein samples by SDS-PAGE and transfer to a membrane.
Antibody incubation: Dilute the antibody 1:1000-10000 in TBST with 5% non-fat dry milk, incubate at 4°C overnight.
Washing or detection: Wash the membrane with TBST, detect using a suitable method.
Technical notes: Optimize blocking conditions to minimize non-specific binding.
Reagents or buffers: Phosphate-buffered saline (PBS), Triton X-100, bovine serum albumin (BSA).
Sample preparation: Fix and embed tissue samples, section into 4-6 μm slices.
Antibody incubation: Dilute the antibody 1:100-1000 in PBS with 1% BSA and 0.1% Triton X-100, incubate at room temperature for 1 hour.
Washing or detection: Wash with PBS, detect using a suitable method.
Technical notes: Antigen retrieval may be necessary for certain tissue samples.
Reagents or buffers: PBS, Triton X-100, BSA.
Sample preparation: Fix tissue samples, permeabilize with 0.1% Triton X-100.
Antibody incubation: Dilute the antibody 1:100-1000 in PBS with 1% BSA, incubate at room temperature for 1 hour.
Washing or detection: Wash with PBS, detect using a suitable fluorescence method.
Technical notes: Use a fluorescence-compatible mounting medium.
Reagents or buffers: PBS, Triton X-100, BSA.
Sample preparation: Fix and permeabilize cells with 0.1% Triton X-100.
Antibody incubation: Dilute the antibody 1:100-1000 in PBS with 1% BSA, incubate at room temperature for 1 hour.
Washing or detection: Wash with PBS, detect using a suitable fluorescence method.
Technical notes: Use a fluorescence-compatible mounting medium.
Reagents or buffers: Coating buffer, blocking buffer, enzyme substrate.
Sample preparation: Coat the plate with antigen, block non-specific binding sites.
Antibody incubation: Dilute the antibody 1:1000-10000 in blocking buffer, incubate at room temperature for 1 hour.
Washing or detection: Wash with PBS, detect using a suitable enzyme substrate.
Technical notes: Optimize coating and blocking conditions to minimize non-specific binding.
Precautions
Store at 2-8°C, handle with care to avoid contamination, optimize antibody dilution for each application, include proper controls to ensure specificity.
Validation Images