Goat Anti-Bovine IgG/HRP
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.1ml | CAB.29535-0.1ml | €37.50 |
| Catalog No | CAB.29535 |
|---|---|
| Product Name | Goat Anti-Bovine IgG/HRP |
| Isotype | N/A |
| Calculated MW | N/A |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Bovine IgG |
| Application | IHC-F;IHC-P;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein G |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat Anti-Bovine IgG/HRP is a versatile antibody suitable for various applications. Below are the protocols for each validated application.
For Western Blotting, dilute the antibody to 1:1000-10000. Prepare samples by lysing cells or tissues in an appropriate buffer, then separate proteins by SDS-PAGE and transfer to a membrane. Incubate the membrane with the diluted antibody for 1-2 hours at room temperature. Wash the membrane with a suitable washing buffer, and detect using an HRP substrate.
For Immunohistochemistry on paraffin sections (IHC-P), dilute the antibody to 1:100-500. Prepare tissue sections by deparaffinization and rehydration. Perform antigen retrieval if necessary. Incubate the sections with the diluted antibody for 30 minutes to 1 hour at room temperature. Wash with a suitable buffer, and detect using an HRP substrate and a chromogen.
For Immunohistochemistry on frozen sections (IHC-F), dilute the antibody to 1:100-1000. Prepare frozen tissue sections and fix them in a suitable fixative. Incubate the sections with the diluted antibody for 30 minutes to 1 hour at room temperature. Wash with a suitable buffer, and detect using an HRP substrate and a chromogen or a fluorescent secondary antibody if necessary.
For Enzyme-Linked Immunosorbent Assay (ELISA), dilute the antibody to 1:1000-10000. Coat ELISA plates with the antigen (bovine IgG) and block non-specific binding sites. Incubate the plates with the diluted antibody for 1-2 hours at room temperature. Wash the plates with a suitable washing buffer, and detect using an HRP substrate.
Technical notes: Optimize the dilution and incubation time for each application as needed. Use appropriate controls to ensure specificity.
Precautions
Store at 2-8°C. Handle with care, and avoid repeated freeze-thaw cycles. Optimize antibody dilution and incubation times for best results.
Validation Images