Goat Anti-Human IgM/APC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
20ul | CAB.29611-20ul | €100.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.29611-50ul | €500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.29611-100ul | €200.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul(PBS Only) | CAB.29611-100ul(PBS Only) | €500.00 |
| Catalog No | CAB.29611 |
|---|---|
| Product Name | Goat Anti-Human IgM/APC |
| Isotype | N/A |
| Calculated MW | 900kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Human IgM |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat Anti-Human IgM/APC antibody is designed for use in various applications, including Immunofluorescence (IF) and Flow Cytometry (FCM). Below are detailed protocols for each validated application.
Reagents or buffers: Goat Anti-Human IgM/APC antibody, phosphate-buffered saline (PBS), 4% paraformaldehyde, and a mounting medium with DAPI.
Sample preparation: Fix cells with 4% paraformaldehyde for 15 minutes, then permeabilize with 0.1% Triton X-100 for 10 minutes.
Antibody incubation: Incubate the cells with the Goat Anti-Human IgM/APC antibody at a dilution of 1:100-1000 in PBS for 1 hour at room temperature.
Washing or detection: Wash the cells three times with PBS, then mount with a mounting medium containing DAPI.
Technical notes: Optimize the antibody dilution and incubation time based on the specific experimental conditions.
Reagents or buffers: Goat Anti-Human IgM/APC antibody, phosphate-buffered saline (PBS), and a flow cytometry buffer.
Sample preparation: Prepare cell suspensions in PBS, then add the Goat Anti-Human IgM/APC antibody at a dilution of 1:100-1000.
Antibody incubation: Incubate the cell suspensions with the antibody for 30 minutes at 4°C.
Washing or detection: Wash the cells twice with PBS, then resuspend in a flow cytometry buffer for analysis.
Technical notes: Use a flow cytometer to detect the APC fluorescence and analyze the data using appropriate software.
Use assay conditions validated for IF, apply Goat Anti-Human IgM/APC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Use assay conditions validated for FCM, apply Goat Anti-Human IgM/APC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Precautions
Store at 2-8°C, handle with care, include proper controls, and optimize antibody dilution and incubation times for best results.
Validation Images