Donkey Anti-Human IgG/HRP
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.29858-100ul | €75.00 |
| Catalog No | CAB.29858 |
|---|---|
| Product Name | Donkey Anti-Human IgG/HRP |
| Isotype | N/A |
| Calculated MW | 160kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Human IgG |
| Application | IHC-F;IHC-P;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Human IgG/HRP is a versatile antibody that can be used in various applications.
Reagents or buffers: Tris-buffered saline with Tween 20 (TBST), skim milk or BSA for blocking, and a chemiluminescent substrate for detection.
Sample preparation: Separate proteins by SDS-PAGE and transfer to a PVDF membrane.
Antibody incubation: Incubate the membrane with the Donkey Anti-Human IgG/HRP at a dilution of 1:1000-10000 in TBST with 5% skim milk or BSA for 1 hour at room temperature.
Washing or detection: Wash the membrane three times with TBST and detect using a chemiluminescent substrate.
Technical notes: Optimize the dilution and incubation time for best results.
Reagents or buffers: Xylene, ethanol, and a peroxidase substrate.
Sample preparation: Fix and embed tissue samples, then section and mount on slides.
Antibody incubation: Incubate the slides with the Donkey Anti-Human IgG/HRP at a dilution of 1:100-500 in a humidified chamber for 30 minutes to 1 hour at room temperature.
Washing or detection: Wash the slides with PBS and detect using a peroxidase substrate.
Technical notes: Use a suitable antigen retrieval method if necessary.
Reagents or buffers: PBS, a fluorescent substrate, and a mounting medium.
Sample preparation: Fix and permeabilize cells or tissue samples.
Antibody incubation: Incubate the samples with the Donkey Anti-Human IgG/HRP at a dilution of 1:100-1000 in PBS for 30 minutes to 1 hour at room temperature.
Washing or detection: Wash the samples with PBS and detect using a fluorescent substrate.
Technical notes: Use a suitable counterstain if necessary.
Reagents or buffers: Coating buffer, blocking buffer, and a substrate for detection.
Sample preparation: Coat the plate with the antigen and block with a blocking buffer.
Antibody incubation: Incubate the plate with the Donkey Anti-Human IgG/HRP at a dilution of 1:1000-10000 in a suitable buffer for 1 hour at room temperature.
Washing or detection: Wash the plate and detect using a substrate.
Technical notes: Optimize the coating and incubation conditions for best results.
Precautions
Store at 2-8°C, handle with care, and use appropriate controls. Optimize antibody dilution and incubation conditions for best results.
Validation Images