Avidin/HRP
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.1ml | CAB.30481-0.1ml | €55.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1ml | CAB.30481-1ml | €375.00 |
| Catalog No | CAB.30481 |
|---|---|
| Product Name | Avidin/HRP |
| Isotype | N/A |
| Calculated MW | N/A |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Avidin |
| Clone Type | N/A |
| Reactivity | Avidin |
| Application | IHC-P;IHC-F;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Avidin/HRP antibody is a versatile tool for detecting avidin in various applications. Below are the protocols for each validated application.
Reagents or buffers: Tris-buffered saline with Tween 20 (TBST), skim milk, and ECL substrate.
Sample preparation: Separate proteins by SDS-PAGE and transfer to a PVDF membrane.
Antibody incubation: Incubate the membrane with Avidin/HRP antibody at a dilution of 1:500-5000 in TBST with 5% skim milk for 1 hour at room temperature.
Washing or detection: Wash the membrane three times with TBST, then detect using ECL substrate.
Technical notes: Optimize the blocking conditions to reduce non-specific binding.
Reagents or buffers: Xylene, ethanol, antigen retrieval buffer, and DAB substrate.
Sample preparation: Fix and embed tissue samples, then section and deparaffinize.
Antibody incubation: Incubate sections with Avidin/HRP antibody at a dilution of 1:400-800 in antibody diluent for 30 minutes at room temperature.
Washing or detection: Wash sections with PBS, then detect using DAB substrate.
Technical notes: Perform antigen retrieval to enhance signal.
Reagents or buffers: Methanol, acetone, and fluorescent secondary antibody.
Sample preparation: Fix cells or tissue sections, then permeabilize with methanol or acetone.
Antibody incubation: Incubate samples with Avidin/HRP antibody at a dilution of 1:400-800 in antibody diluent for 30 minutes at room temperature.
Washing or detection: Wash samples with PBS, then detect using a fluorescent secondary antibody.
Technical notes: Use a fluorescent quencher to reduce background.
Reagents or buffers: Coating buffer, blocking buffer, and TMB substrate.
Sample preparation: Coat plates with avidin antigen, then block with blocking buffer.
Antibody incubation: Incubate plates with Avidin/HRP antibody at a dilution of 1:500-5000 in antibody diluent for 1 hour at room temperature.
Washing or detection: Wash plates with PBS, then detect using TMB substrate.
Technical notes: Optimize the coating and blocking conditions to reduce non-specific binding.
Precautions
Store at 2-8°C, handle with care, include positive and negative controls, and optimize antibody dilution for each application.
Validation Images