Goat Anti-Human IgM/AP
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.30605-100ul | €200.00 |
| Catalog No | CAB.30605 |
|---|---|
| Product Name | Goat Anti-Human IgM/AP |
| Isotype | N/A |
| Calculated MW | 900kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Human IgM |
| Application | IHC-F;IHC-P;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein G |
| Storage Buffer | N/A |
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
Background
IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it’s large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it’s high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences.
Protocol / Instructions
The Goat Anti-Human IgM/AP antibody is designed for use in various applications including Western Blotting, ELISA, Immunohistochemistry on paraffin sections (IHC-P), and Immunohistochemistry on frozen sections (IHC-F).
For Western Blotting, reconstitute the antibody according to the manufacturer's instructions. Prepare samples by lysing cells or tissues in an appropriate buffer, then separate proteins by size using SDS-PAGE. Transfer the proteins to a membrane and block non-specific binding sites with a blocking buffer. Incubate the membrane with the Goat Anti-Human IgM/AP antibody at a dilution of 1:1000-10000 in the blocking buffer. CABter washing, detect the antibody using an alkaline phosphatase substrate. Technical notes: optimize the blocking buffer and washing conditions to minimize background.
For ELISA, coat the plate with the antigen of interest and block non-specific binding sites. Incubate the plate with the Goat Anti-Human IgM/AP antibody at a dilution of 1:1000-10000. CABter washing, detect the antibody using an alkaline phosphatase substrate. Technical notes: optimize the coating and blocking conditions to minimize background.
For IHC-P, deparaffinize and rehydrate tissue sections, then block endogenous peroxidase activity. Incubate the sections with the Goat Anti-Human IgM/AP antibody at a dilution of 1:100-500. CABter washing, detect the antibody using an alkaline phosphatase substrate. Technical notes: optimize the antigen retrieval and blocking conditions to minimize background.
For IHC-F, fix and permeabilize tissue sections, then block non-specific binding sites. Incubate the sections with the Goat Anti-Human IgM/AP antibody at a dilution of 1:100-1000. CABter washing, detect the antibody using an alkaline phosphatase substrate. Technical notes: optimize the fixation and blocking conditions to minimize background.
Reagents or buffers used may vary depending on the specific application and tissue type. Sample preparation is critical for optimal results.
Antibody incubation times and temperatures may need to be optimized for each application.
Precautions
Store at -20°C, handle with care, use appropriate controls, and optimize assay conditions to minimize background and ensure specific binding.
Validation Images