Anti-4E-BP2 Monoclonal Antibody
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.30832-50ul | €272.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.30832-100ul | €437.50 |
| Catalog No | CAB.30832 |
|---|---|
| Product Name | Anti-4E-BP2 Monoclonal Antibody |
| Isotype | IgG1 |
| Calculated MW | 15-20kDa |
| Immunogen | Recombinant protein of human 4E-BP2 |
| Public Immunogen Range | N/A |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Reactivity | Mouse;Human;Rat |
| Application | WB |
| Subcellular Location | Cytosol Nucleus |
| Purification Method | CABfinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，0.5% BSA，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
Background
Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase pathway.
Protocol / Instructions
The Anti-4E-BP2 Monoclonal Antibody is a high-quality antibody that can be used for Western Blotting (WB) applications.
Reagents or buffers required include the Anti-4E-BP2 Monoclonal Antibody, blocking buffer, washing buffer, and a detection system.
Sample preparation involves lysing cells or tissues in an appropriate lysis buffer, followed by protein quantification and separation by SDS-PAGE.
Antibody incubation is performed by adding the Anti-4E-BP2 Monoclonal Antibody at a recommended dilution of 1:500-1000 to the membrane and incubating overnight at 4°C.
Washing is done using a washing buffer to remove excess antibody, and detection is achieved using a suitable detection system.
Technical notes: Optimize the antibody dilution and incubation time for best results, and use a suitable positive control to verify the specificity of the antibody.
Precautions
Store at -20°C, handle with care, and optimize antibody dilution for best results.
Validation Images