Goat Anti-Human IgG-Fc/RBITC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
300ul | CAB.31233-300ul | €45.00 |
| Catalog No | CAB.31233 |
|---|---|
| Product Name | Goat Anti-Human IgG-Fc/RBITC |
| Isotype | N/A |
| Calculated MW | N/A |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Human IgG-Fc |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat Anti-Human IgG-Fc/RBITC antibody is a valuable tool for various immunofluorescence (IF) applications. Below are the protocols for the validated application key provided.
For IF applications, the following steps can be followed:
Reagents or buffers: Phosphate-buffered saline (PBS), 4% paraformaldehyde for fixation, 0.1% Triton X-100 for permeabilization, and a blocking buffer containing 5% normal serum.
Sample preparation: Fix cells or tissue sections with 4% paraformaldehyde, followed by permeabilization with 0.1% Triton X-100. Block non-specific binding sites with a blocking buffer.
Antibody incubation: Incubate the sample with the Goat Anti-Human IgG-Fc/RBITC antibody at a dilution of 1:50-500 in the blocking buffer for 1-2 hours at room temperature or overnight at 4°C.
Washing or detection: Wash the sample thoroughly with PBS, and then detect the signal using a fluorescence microscope.
Technical notes: Optimize the antibody dilution and incubation time based on the specific application and sample type.
Use assay conditions validated for IF, apply Goat Anti-Human IgG-Fc/RBITC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:50-500.
Precautions
Store at 2-8°C, handle with care, and optimize antibody dilution and incubation time for best results.
Validation Images