Donkey Anti-Goat IgG/PE-CY5
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.31762-100ul | €200.00 |
| Catalog No | CAB.31762 |
|---|---|
| Product Name | Donkey Anti-Goat IgG/PE-CY5 |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Goat IgG/PE-CY5 is a versatile antibody conjugate suitable for various applications, including Immunofluorescence (IF) and Flow Cytometry (FCM). Below are the protocols for each validated application.
For IF, the following steps are recommended:
- Reagents or Buffers: Phosphate-buffered saline (PBS), 4% paraformaldehyde for fixation, and a mounting medium with DAPI for nuclear staining.
- Sample Preparation: Fix cells or tissue sections with 4% paraformaldehyde, permeabilize if necessary, and block with a suitable blocking buffer.
- Antibody Incubation: Dilute the Donkey Anti-Goat IgG/PE-CY5 to 1:100-1000 in the blocking buffer and incubate for 1-2 hours at room temperature or overnight at 4°C.
- Washing or Detection: Wash samples thoroughly with PBS, and proceed with mounting and imaging.
- Technical Notes: Optimize the dilution and incubation time based on the specific experimental conditions and the intensity of the fluorescence signal.
For FCM, follow these guidelines:
- Reagents or Buffers: PBS, FACS buffer (PBS with 1% BSA and 0.1% sodium azide), and a suitable fixation buffer if needed.
- Sample Preparation: Prepare cell suspensions and block with FACS buffer if necessary.
- Antibody Incubation: Dilute the Donkey Anti-Goat IgG/PE-CY5 to 1:100-1000 in FACS buffer and incubate for 30 minutes to 1 hour on ice.
- Washing or Detection: Wash cells with FACS buffer, and analyze by flow cytometry.
- Technical Notes: Compensation for the PE-CY5 channel may be required depending on the flow cytometer and other fluorophores used. Optimize the antibody dilution and incubation time for the best signal-to-noise ratio.
Use assay conditions validated for IF, apply Donkey Anti-Goat IgG/PE-CY5 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Use assay conditions validated for FCM, apply Donkey Anti-Goat IgG/PE-CY5 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Precautions
Store at 4°C, handle with care, and optimize antibody dilutions for each application to ensure specificity and sensitivity.
Validation Images