Donkey Anti-Goat IgG
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mg | CAB.33127-1mg | €27.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
10mg | CAB.33127-10mg | €1562.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100mg | CAB.33127-100mg | €1562.50 |
| Catalog No | CAB.33127 |
|---|---|
| Product Name | Donkey Anti-Goat IgG |
| Isotype | IgG |
| Calculated MW | 150kDa |
| Immunogen | Goat IgG full length protein |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | Polyclonal Antibody |
| Reactivity | Goat |
| Application | ELISA WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purification |
| Storage Buffer | Buffer: PBS with 0.03% Proclin300 （PH7.2-7.4). |
| Storage | Shipped at 4℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Immunoglobulin G (IgG)，is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions;to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Protocol / Instructions
The Donkey Anti-Goat IgG is a versatile antibody that can be used in various applications, including ELISA and Western Blotting.
For ELISA, the recommended dilution is 1:5000-100000. The reagents required include the Donkey Anti-Goat IgG, goat IgG antigen, and ELISA substrate. Sample preparation involves coating the ELISA plate with the antigen, followed by blocking with a suitable blocking buffer. The Donkey Anti-Goat IgG is then incubated with the antigen, and the plate is washed with a washing buffer. The signal is detected using an ELISA substrate, and the absorbance is measured using an ELISA reader. Technical notes: it is essential to optimize the concentration of the antigen and the antibody to achieve the best results.
For Western Blotting, the recommended dilution is 1:1000-20000. The reagents required include the Donkey Anti-Goat IgG, SDS-PAGE gel, and Western Blotting substrate. Sample preparation involves separating the proteins using SDS-PAGE, followed by transferring the proteins to a membrane. The membrane is then blocked with a suitable blocking buffer, and the Donkey Anti-Goat IgG is incubated with the membrane. The membrane is washed with a washing buffer, and the signal is detected using a Western Blotting substrate. Technical notes: it is crucial to optimize the concentration of the antibody and the blocking buffer to achieve the best results.
Use assay conditions validated for ELISA WB, apply Donkey Anti-Goat IgG at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: WB 1:1000-20000.;ELISA:1:5000-100000..
Precautions
Store at 2-8°C. Handle with care, and avoid repeated freeze-thaw cycles. Use appropriate controls and optimize antibody dilution for best results.
Validation Images