Goat Anti-Bovine IgG/AP
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.33828-100ul | €87.50 |
| Catalog No | CAB.33828 |
|---|---|
| Product Name | Goat Anti-Bovine IgG/AP |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Bovine IgG |
| Application | IHC-F;IHC-P;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat Anti-Bovine IgG/AP antibody is designed for use in various applications including Western Blotting, ELISA, Immunohistochemistry on paraffin sections (IHC-P), and Immunohistochemistry on frozen sections (IHC-F).
For Western Blotting, reconstitute the antibody in the provided buffer and dilute to the recommended concentration of 1:1000-10000 in a suitable buffer such as TBS or PBS with 0.1% Tween-20. Sample preparation involves separating proteins by SDS-PAGE and transferring them to a membrane. The membrane is then blocked with a blocking buffer and incubated with the diluted antibody. CABter washing, the membrane is incubated with a suitable alkaline phosphatase substrate to detect the target protein. Technical notes: Optimize the blocking conditions and antibody dilution to minimize background and enhance specific signal.
For IHC-P, the antibody is used at a dilution of 1:100-1000. Sample preparation involves fixing and embedding tissue samples in paraffin, followed by sectioning. The sections are then deparaffinized and rehydrated before antigen retrieval. The diluted antibody is applied to the sections, followed by washing and detection using an alkaline phosphatase substrate. Technical notes: The choice of antigen retrieval method may CABfect the staining results.
For IHC-F, the antibody is used at a dilution of 1:100-1000. Sample preparation involves fixing frozen tissue sections in a suitable fixative. The sections are then blocked and incubated with the diluted antibody. CABter washing, the sections are incubated with a suitable alkaline phosphatase substrate to detect the target protein. Technical notes: The choice of fixative and fixation time may CABfect the staining results.
For ELISA, the antibody is used at a dilution of 1:1000-10000. Sample preparation involves coating the wells of a microtiter plate with the antigen. The diluted antibody is then added to the wells, followed by washing and detection using an alkaline phosphatase substrate. Technical notes: Optimize the coating concentration and antibody dilution to minimize background and enhance specific signal.
Precautions
Store at -20°C, handle with care, include positive and negative controls, and optimize antibody dilution and reaction conditions to minimize background and enhance specific signal.
Validation Images