Donkey Anti-Goat IgG（Control-line）
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mg | CAB.34243-1mg | €55.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
10mg | CAB.34243-10mg | €3125.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100mg | CAB.34243-100mg | €3125.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
20T | CAB.34243-20T | €302.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100T | CAB.34243-100T | €720.00 |
| Catalog No | CAB.34243 |
|---|---|
| Product Name | Donkey Anti-Goat IgG（Control-line） |
| Isotype | IgG |
| Calculated MW | 150kDa |
| Immunogen | Goat IgG full length protein |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | Polyclonal Antibody |
| Reactivity | Goat |
| Application | ELISA;WB;LFIA |
| Subcellular Location | N/A |
| Purification Method | CABfinity purification |
| Storage Buffer | PBS with 0.03% Proclin300（PH7.2-7.4). |
| Storage | Store at -20℃，at least 1 year.(Avoid freeze/thaw cycles). |
Background
Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Protocol / Instructions
The Donkey Anti-Goat IgG antibody is designed for various applications including ELISA, Western Blotting, and Lateral Flow Immunoassay.
For ELISA, the recommended dilution is 1:5000-100000. The reagents required include the Donkey Anti-Goat IgG antibody, goat IgG antigen, ELISA plates, and detection reagents. Sample preparation involves coating the ELISA plates with the goat IgG antigen. The antibody incubation step involves adding the Donkey Anti-Goat IgG antibody at the recommended dilution. Washing and detection involve using a detection reagent that reacts with the Donkey Anti-Goat IgG antibody. Technical notes: Optimize the antibody dilution and incubation time for best results.
For Western Blotting, the recommended dilution is 1:1000-20000. The reagents required include the Donkey Anti-Goat IgG antibody, SDS-PAGE gel, transfer membrane, and detection reagents. Sample preparation involves separating the proteins by SDS-PAGE and transferring them to a membrane. The antibody incubation step involves adding the Donkey Anti-Goat IgG antibody at the recommended dilution. Washing and detection involve using a detection reagent that reacts with the Donkey Anti-Goat IgG antibody. Technical notes: Use a suitable blocking buffer to reduce non-specific binding.
For Lateral Flow Immunoassay, the recommended concentration for the control line is 0.25-2.5 mg/ml. The reagents required include the Donkey Anti-Goat IgG antibody, LFIA strips, and sample preparation reagents. Sample preparation involves preparing the sample according to the LFIA kit instructions. The antibody incubation step involves adding the sample to the LFIA strip. Washing and detection involve using the LFIA kit reagents. Technical notes: Follow the LFIA kit instructions for best results.
Precautions
Store at 2-8°C. Handle with care, avoid repeated freeze-thaw cycles. Use suitable controls and optimize antibody dilution for best results.
Validation Images