Donkey Anti-Goat IgG/HRP
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.34377-100ul | €27.50 |
| Catalog No | CAB.34377 |
|---|---|
| Product Name | Donkey Anti-Goat IgG/HRP |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | IHC-F;IHC-P;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Goat IgG/HRP is a versatile antibody that can be used in various applications.
For optimal results, it is essential to follow the recommended dilutions and protocols for each application.
Reagents or buffers: Tris-buffered saline with Tween 20 (TBST), skim milk or BSA for blocking, and a suitable substrate for HRP detection.
Sample preparation: Prepare protein samples and separate them by SDS-PAGE. Transfer the proteins to a membrane and block with skim milk or BSA.
Antibody incubation: Incubate the membrane with the Donkey Anti-Goat IgG/HRP at a dilution of 1:1000-10000 in TBST for 1-2 hours at room temperature.
Washing or detection: Wash the membrane with TBST and detect the signal using a suitable substrate for HRP.
Reagents or buffers: Phosphate-buffered saline (PBS), normal donkey serum for blocking, and a suitable chromogen for detection.
Sample preparation: Prepare paraffin-embedded tissue sections and deparaffinize them. Antigen retrieval may be necessary.
Antibody incubation: Incubate the sections with the Donkey Anti-Goat IgG/HRP at a dilution of 1:100-1000 in PBS for 30 minutes to 1 hour at room temperature.
Washing or detection: Wash the sections with PBS and detect the signal using a suitable chromogen.
Reagents or buffers: PBS, normal donkey serum for blocking, and a suitable fluorophore for detection.
Sample preparation: Prepare frozen tissue sections and fix them with a suitable fixative. Permeabilize the sections if necessary.
Antibody incubation: Incubate the sections with the Donkey Anti-Goat IgG/HRP at a dilution of 1:100-1000 in PBS for 30 minutes to 1 hour at room temperature.
Washing or detection: Wash the sections with PBS and detect the signal using a suitable fluorophore.
Reagents or buffers: Coating buffer, blocking buffer, and a suitable substrate for HRP detection.
Sample preparation: Coat the ELISA plate with the antigen and block with a suitable blocking buffer.
Antibody incubation: Incubate the plate with the Donkey Anti-Goat IgG/HRP at a dilution of 1:1000-10000 in the blocking buffer for 1-2 hours at room temperature.
Washing or detection: Wash the plate and detect the signal using a suitable substrate for HRP.
Technical notes: Optimize the antibody dilution and incubation time for each application to achieve the best results. Use suitable controls to ensure the specificity of the antibody.
Precautions
Store at 2-8°C. Handle with care, and avoid repeated freeze-thaw cycles. Use suitable controls and optimize the antibody dilution and incubation time for each application.
Validation Images