Anti-ACTA1 Recombinant Antibody
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.35187-50ul | €125.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.35187-100ul | €210.00 |
| Catalog No | CAB.35187 |
|---|---|
| Product Name | Anti-ACTA1 Recombinant Antibody |
| Isotype | IgG |
| Calculated MW | N/A |
| Immunogen | A synthetic peptide of human ACTA1 |
| Public Immunogen Range | N/A |
| Host | Rabbit |
| Clone Type | Recombinant Antibody |
| Reactivity | Human;Mouse;Rat |
| Application | WB;IF/ICC;FCM |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | PBS (pH 7.4) containing 50% glycerol，and 0.02% sodium azide. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
Background
The product encoded by this gene belongs to the actin family of proteins，which are highly conserved proteins that play a role in cell motility，structure and integrity. Alpha，beta and gamma actin isoforms have been identified，with alpha actins being a major constituent of the contractile apparatus，while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3，congenital myopathy with excess of thin myofilaments，congenital myopathy with cores，and congenital myopathy with fiber-type disproportion，diseases that lead to muscle fiber defects.
Protocol / Instructions
The Anti-ACTA1 Recombinant Antibody is a rabbit-derived antibody that recognizes the ACTA1 protein in human, mouse, and rat samples. This antibody has been validated for use in Western Blotting (WB), Immunofluorescence/Immunocytochemistry (IF/ICC), and Flow Cytometry (FCM) applications.
For Western Blotting, the following reagents and buffers are recommended: RIPA buffer for cell lysis, SDS-PAGE gel for protein separation, and PVDF membrane for protein transfer. Sample preparation involves lysing cells in RIPA buffer, followed by centrifugation and boiling in SDS sample buffer. The antibody should be incubated at a suitable dilution (to be determined by the user) in TBST buffer with 5% non-fat milk overnight at 4°C. Washing should be performed with TBST buffer, and detection can be achieved using an HRP-conjugated secondary antibody and ECL substrate. Technical notes: the expected band size is approximately 42 kDa, and the antibody may cross-react with other actin isoforms.
For Immunofluorescence/Immunocytochemistry, the following reagents and buffers are recommended: 4% paraformaldehyde for cell fixation, 0.1% Triton X-100 for permeabilization, and PBS buffer for washing. Sample preparation involves fixing and permeabilizing cells, followed by blocking with 5% non-fat milk in PBS. The antibody should be incubated at a suitable dilution (to be determined by the user) in PBS with 1% BSA for 1 hour at room temperature. Washing should be performed with PBS, and detection can be achieved using a fluorescently labeled secondary antibody. Technical notes: the antibody may require optimization of fixation and permeabilization conditions for optimal staining.
For Flow Cytometry, the following reagents and buffers are recommended: PBS buffer with 1% BSA for washing and antibody incubation, and a fixative such as 4% paraformaldehyde for cell fixation. Sample preparation involves fixing and washing cells, followed by incubation with the antibody at a suitable dilution (to be determined by the user) in PBS with 1% BSA for 30 minutes at 4°C. Washing should be performed with PBS, and detection can be achieved using a fluorescently labeled secondary antibody. Technical notes: the antibody may require optimization of fixation and permeabilization conditions for optimal staining, and compensation may be necessary to account for spectral overlap.
Precautions
Store at -20°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation conditions for each application to ensure specific and sensitive detection of the target protein.
Validation Images