CATEGORIES
Donkey Anti-Goat IgG/AP
Product classification
Antibodies
Secondary Antibodies
Catalog No
CAB.37767
Antibody overview
Ordering
Available Variants20ul
CAB.37767-20ul
Price
€100.00
Available – Dispatch in 14 days
50ul
CAB.37767-50ul
Price
€500.00
Available – Dispatch in 14 days
100ul
CAB.37767-100ul
Price
€87.50
Available – Dispatch in 14 days
100ul(PBS Only)
CAB.37767-100ul(PBS Only)
Price
€500.00
Available – Dispatch in 14 days
| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
20ul | CAB.37767-20ul | €100.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.37767-50ul | €500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.37767-100ul | €87.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul(PBS Only) | CAB.37767-100ul(PBS Only) | €500.00 |
Quick page guide: Compare pack sizes, review validated applications and access product documents from this section.
Recommended Dilution
Dilution
IHC-F=1:100-1000
Validated Application Key
IHC-F
Validated application
IHC-F - Immunohistochemistry (Frozen sections)
IHC-P
Validated application
IHC-P - Immunohistochemistry (Paraffin-embedded sections)
ELISA
Validated application
ELISA - Enzyme-Linked Immunosorbent Assay
WB
Validated application
WB - Western Blotting
Technical Specifications
| Catalog No | CAB.37767 |
|---|---|
| Product Name | Donkey Anti-Goat IgG/AP |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | IHC-F;IHC-P;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
Recommended workflow
Optimized handling and experimental guidance
Review the suggested sequence below before starting the assay to keep sample handling, incubation, and downstream interpretation aligned.
Lab-ready steps
**IHC-F Protocol**
- Use fresh frozen sections fixed briefly with cold acetone or paraformaldehyde according to antigen sensitivity.
- Bring slides to room temperature, wash in PBS, and block nonspecific binding before primary antibody incubation.
- Apply Donkey Anti-Goat IgG/AP at the optimized dilution for 1 hour at room temperature or overnight at 4°C. Recommended dilution: WB=1:1000-10000;
- Wash in PBS or TBS, apply enzyme- or fluorophore-conjugated secondary antibody, and protect from light when fluorescence detection is used.
- Mount with compatible medium and evaluate signal localization against isotype or no-primary control sections.
ELISA=1:1000-10000;
IHC-P=1:100-1000;
IHC-F=1:100-1000.
**IHC-P Protocol**
- Use formalin-fixed, paraffin-embedded sections mounted on charged slides. Bake, deparaffinize in xylene, and rehydrate through graded ethanol to water.
- Perform heat-induced epitope retrieval in citrate or EDTA buffer, then cool and rinse in PBS or TBS.
- Quench endogenous peroxidase if chromogenic detection will be used, and block nonspecific binding with serum or protein block for 20–30 minutes.
- Incubate sections with Donkey Anti-Goat IgG/AP in antibody diluent for 1 hour at room temperature or overnight at 4°C. Recommended dilution: WB=1:1000-10000;
- Wash thoroughly, apply the appropriate secondary detection system, develop with DAB or equivalent substrate, counterstain with hematoxylin, dehydrate, mount, and compare specific staining with negative control tissue.
ELISA=1:1000-10000;
IHC-P=1:100-1000;
IHC-F=1:100-1000.
**ELISA Protocol**
- Coat the plate with antigen or capture reagent according to assay design, block with protein-containing buffer to reduce nonspecific binding, and incubate with Donkey Anti-Goat IgG/AP at the optimized working dilution. Recommended dilution: WB=1:1000-10000;
- Wash between each incubation using PBS or TBS containing a low concentration of Tween-20.
- Add enzyme-conjugated secondary antibody, develop with appropriate substrate, stop the reaction at the recommended endpoint, and read absorbance promptly.
- Generate a control-based interpretation using blank, negative, and positive wells.
ELISA=1:1000-10000;
IHC-P=1:100-1000;
IHC-F=1:100-1000.
**Western Blotting Protocol**
- Prepare lysates in SDS-compatible lysis buffer containing protease inhibitors, clarify by centrifugation, and denature samples at 95–100°C for 5 minutes.
- Resolve proteins by SDS-PAGE and transfer to PVDF or nitrocellulose membrane.
- Block with 5% BSA or nonfat milk in TBST for 1 hour at room temperature.
- Incubate the membrane with Donkey Anti-Goat IgG/AP diluted in antibody dilution buffer overnight at 4°C with gentle agitation. Recommended dilution: WB=1:1000-10000;
- Wash 3 times in TBST, then incubate with species-appropriate HRP-conjugated secondary antibody for 1 hour at room temperature.
- Wash again, develop with ECL substrate, and document target band intensity together with molecular weight marker and exposure conditions.
ELISA=1:1000-10000;
IHC-P=1:100-1000;
IHC-F=1:100-1000.
Precautions
For research use only. Store under the recommended conditions and avoid repeated freeze-thaw cycles. Mix gently before use, centrifuge briefly if needed, and validate dilution, retrieval, fixation, permeabilization, and incubation conditions in your assay system. Use appropriate positive, negative, and no-primary controls, and handle all biological samples using standard laboratory biosafety procedures.
Validation Images
Fig.1.
Validation data.