Donkey Anti-Goat IgG/Solar Fluor 405
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.38438-100ul | €87.50 |
| Catalog No | CAB.38438 |
|---|---|
| Product Name | Donkey Anti-Goat IgG/Solar Fluor 405 |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Goat IgG/Solar Fluor 405 is a secondary antibody conjugated to Solar Fluor 405, suitable for various applications including Immunofluorescence (IF).
Reagents or buffers: Phosphate-buffered saline (PBS), Solar Fluor 405 conjugated secondary antibody, mounting medium.
Sample preparation: Fix cells or tissue sections with a suitable fixative, permeabilize if necessary, and block non-specific binding sites with a blocking buffer.
Antibody incubation: Incubate the sample with the primary antibody, followed by incubation with the Donkey Anti-Goat IgG/Solar Fluor 405 at a dilution of 1:50-500.
Washing or detection: Wash the sample thoroughly CABter primary and secondary antibody incubations, and detect the signal using a fluorescence microscope.
Technical notes: Optimize the dilution of the secondary antibody and the incubation time to achieve the best signal-to-noise ratio.
Use assay conditions validated for IF, apply Donkey Anti-Goat IgG/Solar Fluor 405 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:50-500.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution for best results.
Validation Images