Goat anti-Mouse IgG
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mg | CAB.38603-1mg | €27.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
10mg | CAB.38603-10mg | €1562.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100mg | CAB.38603-100mg | €1562.50 |
| Catalog No | CAB.38603 |
|---|---|
| Product Name | Goat anti-Mouse IgG |
| Isotype | IgG |
| Calculated MW | 150kDa |
| Immunogen | Mouse IgG full length protein |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | Polyclonal Antibody |
| Reactivity | Mouse |
| Application | WB;ELISA |
| Subcellular Location | N/A |
| Purification Method | CABfinity purification |
| Storage Buffer | Buffer: PBS with 0.03% Proclin300（PH7.2-7.4). |
| Storage | Shipped at 4℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Immunoglobulin G (IgG)，is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions;to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Protocol / Instructions
The Goat anti-Mouse IgG antibody is designed for use in various applications including Western Blotting (WB) and Enzyme-Linked Immunosorbent Assay (ELISA).
Reagents or buffers: Tris-buffered saline with Tween 20 (TBST) as the washing buffer, and a blocking buffer such as 5% non-fat dry milk in TBST.
Sample preparation: Prepare the protein samples by lysing cells or tissues in an appropriate lysis buffer, followed by separation using SDS-PAGE and transfer to a membrane.
Antibody incubation: Incubate the membrane with the Goat anti-Mouse IgG antibody at the recommended dilution in blocking buffer for 1-2 hours at room temperature or overnight at 4°C.
Washing or detection: Wash the membrane with TBST, and then detect using a suitable detection system such as chemiluminescence.
Technical notes: Optimize the blocking conditions and antibody dilution to minimize background and enhance specific signal.
Reagents or buffers: A coating buffer such as carbonate-bicarbonate buffer, a blocking buffer such as 1% bovine serum albumin in PBS, and a washing buffer such as PBS with Tween 20.
Sample preparation: Prepare the antigen by coating it onto the ELISA plate using the coating buffer.
Antibody incubation: Incubate the plate with the Goat anti-Mouse IgG antibody at the recommended dilution in blocking buffer for 1-2 hours at room temperature.
Washing or detection: Wash the plate with washing buffer, and then detect using a suitable detection system such as a chromogenic substrate.
Technical notes: Optimize the coating conditions, antibody dilution, and detection system to achieve the best results.
The Goat anti-Mouse IgG antibody is expected to react with Mouse IgG due to its immunogen being Mouse IgG full length protein. It is essential to handle the antibody with care and follow the recommended storage conditions to maintain its activity and specificity.
Precautions
Store at -20°C, handle with care, and optimize antibody dilution and experimental conditions to avoid non-specific binding and ensure accurate results.
Validation Images