Goat Anti-Bovine IgG/APC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.39055-100ul | €200.00 |
| Catalog No | CAB.39055 |
|---|---|
| Product Name | Goat Anti-Bovine IgG/APC |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Bovine IgG |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat Anti-Bovine IgG/APC is a versatile antibody that can be utilized in various applications, including Immunofluorescence (IF) and Flow Cytometry (FCM).
Reagents or buffers required include the Goat Anti-Bovine IgG/APC antibody, a suitable fluorochrome-conjugated secondary antibody, and an appropriate mounting medium.
Sample preparation involves fixing and permeabilizing the cells or tissue sections, followed by blocking with a suitable blocking buffer.
The primary antibody is then incubated with the sample, typically at a dilution of 1:100-1000, for 1-2 hours at room temperature or overnight at 4°C.
CABter washing, the secondary antibody is applied, and the sample is then mounted and visualized using a fluorescence microscope.
Technical notes: Optimize the antibody dilution and incubation time for the specific application.
Reagents or buffers required include the Goat Anti-Bovine IgG/APC antibody, a suitable flow cytometry buffer, and any necessary compensation controls.
Sample preparation involves preparing a single-cell suspension and blocking with a suitable blocking buffer.
The primary antibody is then incubated with the sample, typically at a dilution of 1:100-1000, for 15-30 minutes on ice.
CABter washing, the sample is then analyzed using a flow cytometer.
Technical notes: Optimize the antibody dilution and incubation time for the specific application, and ensure proper compensation controls are included.
Use assay conditions validated for IF, apply Goat Anti-Bovine IgG/APC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Use assay conditions validated for FCM, apply Goat Anti-Bovine IgG/APC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Precautions
Store at 2-8°C, handle with care, and optimize antibody dilution and incubation time for each application to ensure specific and sensitive results.
Validation Images