Goat anti-Chicken IgG/Bio
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.39367-100ul | €35.00 |
| Catalog No | CAB.39367 |
|---|---|
| Product Name | Goat anti-Chicken IgG/Bio |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Chicken IgG |
| Application | IF;IHC-F;IHC-P;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein G |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat anti-Chicken IgG/Bio antibody is a versatile tool for detecting Chicken IgG in various applications. Below are the protocols for each validated application.
Reagents or buffers: Tris-buffered saline with Tween 20 (TBST), skim milk, and Goat anti-Chicken IgG/Bio antibody.
Sample preparation: Separate proteins by SDS-PAGE and transfer to a PVDF membrane.
Antibody incubation: Incubate the membrane with the Goat anti-Chicken IgG/Bio antibody at a dilution of 1:1000-10000 in TBST with 5% skim milk for 1 hour at room temperature.
Washing or detection: Wash the membrane three times with TBST and detect using a suitable detection system.
Reagents or buffers: Phosphate-buffered saline (PBS), normal serum, and Goat anti-Chicken IgG/Bio antibody.
Sample preparation: Fix and paraffin-embed tissue samples, then section and mount on slides.
Antibody incubation: Incubate the sections with the Goat anti-Chicken IgG/Bio antibody at a dilution of 1:100-1000 in PBS with 1% normal serum for 1 hour at room temperature.
Washing or detection: Wash the sections three times with PBS and detect using a suitable detection system.
Reagents or buffers: PBS, normal serum, and Goat anti-Chicken IgG/Bio antibody.
Sample preparation: Fix and permeabilize tissue samples, then section and mount on slides.
Antibody incubation: Incubate the sections with the Goat anti-Chicken IgG/Bio antibody at a dilution of 1:100-1000 in PBS with 1% normal serum for 1 hour at room temperature.
Washing or detection: Wash the sections three times with PBS and detect using a suitable detection system.
Reagents or buffers: PBS, normal serum, and Goat anti-Chicken IgG/Bio antibody.
Sample preparation: Fix and permeabilize cells or tissue samples, then mount on slides.
Antibody incubation: Incubate the samples with the Goat anti-Chicken IgG/Bio antibody at a dilution of 1:100-1000 in PBS with 1% normal serum for 1 hour at room temperature.
Washing or detection: Wash the samples three times with PBS and detect using a suitable fluorescence detection system.
Reagents or buffers: Coating buffer, blocking buffer, and Goat anti-Chicken IgG/Bio antibody.
Sample preparation: Coat the ELISA plate with the antigen and block with a suitable blocking buffer.
Antibody incubation: Incubate the plate with the Goat anti-Chicken IgG/Bio antibody at a dilution of 1:1000-10000 in the blocking buffer for 1 hour at room temperature.
Washing or detection: Wash the plate three times with a washing buffer and detect using a suitable detection system.
Technical notes: Optimize the antibody dilution and incubation time for each application to achieve the best results.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation time for each application.
Validation Images