Goat anti-Mouse IgG（Control-line）
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mg | CAB.39381-1mg | €55.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
10mg | CAB.39381-10mg | €3125.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100mg | CAB.39381-100mg | €3125.00 |
| Catalog No | CAB.39381 |
|---|---|
| Product Name | Goat anti-Mouse IgG（Control-line） |
| Isotype | IgG |
| Calculated MW | 150kDa |
| Immunogen | Mouse IgG full length protein |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | Polyclonal Antibody |
| Reactivity | Mouse |
| Application | ELISA WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purification |
| Storage Buffer | Buffer: 2.5mM Na?CO? with 0.1% NaN?. |
| Storage | Shipped at 4℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Immunoglobulin G (IgG)，is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions;to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Protocol / Instructions
The Goat anti-Mouse IgG antibody is designed for use in various applications including ELISA and Western Blotting.
For ELISA, the recommended dilution is 1:5000-100000. Reagents and buffers required include the antigen of interest, coating buffer, blocking buffer, and substrate. Sample preparation involves coating the antigen onto the plate, followed by blocking and addition of the diluted antibody. Antibody incubation is typically 1-2 hours at room temperature. Washing is performed with a mild detergent, and detection is achieved using a suitable substrate. Technical notes: Optimize the coating and blocking conditions to minimize non-specific binding.
For Western Blotting, the recommended dilution is 1:10000-100000. Reagents and buffers required include sample buffer, running buffer, transfer buffer, and blocking buffer. Sample preparation involves mixing the sample with sample buffer and heating, followed by SDS-PAGE separation and transfer to a membrane. Antibody incubation is typically overnight at 4°C. Washing is performed with a mild detergent, and detection is achieved using a suitable substrate. Technical notes: Optimize the transfer conditions to ensure efficient transfer of the target protein.
Use assay conditions validated for ELISA WB, apply Goat anti-Mouse IgG（Control-line） at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: WB 1:10000-100000.;ELISA:1:5000-100000..
Precautions
Store at -20°C, handle with care, include proper controls, and optimize antibody dilution and incubation conditions to minimize non-specific binding.
Validation Images