BSA/Solar Fluor 405
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.41620-100ul | €87.50 |
| Catalog No | CAB.41620 |
|---|---|
| Product Name | BSA/Solar Fluor 405 |
| Isotype | N/A |
| Calculated MW | 66kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | BSA |
| Clone Type | N/A |
| Reactivity | BSA |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The BSA/Solar Fluor 405 antibody is a valuable tool for detecting Bovine Serum Albumin (BSA) in various applications.
For IF, start by preparing your samples. Fix cells or tissue sections with a suitable fixative, such as paraformaldehyde, and permeabilize if necessary.
Use a blocking buffer to reduce non-specific binding.
Dilute the BSA/Solar Fluor 405 antibody to 1:50-500 in an appropriate diluent, considering the recommended dilution for IF.
Incubate the antibody with your sample, followed by washing steps to remove excess antibody.
Detect the signal using a fluorescence microscope, considering the excitation and emission properties of Solar Fluor 405.
Technical notes: Optimize the antibody dilution and incubation time based on your specific experimental conditions and sample type.
Use assay conditions validated for IF, apply BSA/Solar Fluor 405 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:50-500.
Precautions
Store at 2-8°C. Handle with care, avoiding freeze-thaw cycles. Use appropriate controls and optimize antibody dilution for best results.
Validation Images