Donkey Anti-Goat IgG/Cy3.5
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.42030-100ul | €67.50 |
| Catalog No | CAB.42030 |
|---|---|
| Product Name | Donkey Anti-Goat IgG/Cy3.5 |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Goat IgG/Cy3.5 antibody is a valuable tool for various applications, including Immunofluorescence (IF).
Reagents or buffers: phosphate-buffered saline (PBS), mounting medium, and a fluorescence microscope.
Sample preparation: Fix cells or tissue sections with a suitable fixative, such as paraformaldehyde, and permeabilize with a detergent like Triton X-100 if necessary.
Antibody incubation: Dilute the Donkey Anti-Goat IgG/Cy3.5 antibody to 1:50-500 in PBS and incubate with the sample for 1-2 hours at room temperature or overnight at 4°C.
Washing or detection: Wash the sample with PBS and detect the fluorescence signal using a fluorescence microscope.
Technical notes: Optimize the antibody dilution and incubation time for the specific application and sample type.
Use assay conditions validated for IF, apply Donkey Anti-Goat IgG/Cy3.5 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:50-500.
Precautions
Store at 2-8°C, handle with care, and optimize antibody dilution and incubation time for best results.
Validation Images