Goat Anti-Human IgG/APC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.42767-100ul | €200.00 |
| Catalog No | CAB.42767 |
|---|---|
| Product Name | Goat Anti-Human IgG/APC |
| Isotype | N/A |
| Calculated MW | 160kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Human IgG |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat Anti-Human IgG/APC antibody is designed for use in various applications, including Immunofluorescence (IF) and Flow Cytometry (FCM).
Reagents or buffers: phosphate-buffered saline (PBS), 4% paraformaldehyde, 0.1% Triton X-100, and a mounting medium with DAPI.
Sample preparation: fix cells with 4% paraformaldehyde for 15 minutes, then permeabilize with 0.1% Triton X-100 for 10 minutes.
Antibody incubation: incubate the sample with the Goat Anti-Human IgG/APC antibody at a dilution of 1:100-1000 in PBS for 1 hour at room temperature.
Washing or detection: wash the sample three times with PBS, then mount with a mounting medium containing DAPI.
Technical notes: use a secondary antibody if necessary, and optimize the antibody dilution as needed.
Reagents or buffers: phosphate-buffered saline (PBS), 1% bovine serum albumin (BSA), and a fixative such as 1% paraformaldehyde.
Sample preparation: prepare cell suspensions in PBS, then block with 1% BSA for 10 minutes.
Antibody incubation: incubate the sample with the Goat Anti-Human IgG/APC antibody at a dilution of 1:100-1000 in PBS for 30 minutes on ice.
Washing or detection: wash the sample twice with PBS, then fix with 1% paraformaldehyde and analyze by flow cytometry.
Technical notes: use a compensation control to adjust for spectral overlap, and optimize the antibody dilution as needed.
Use assay conditions validated for IF, apply Goat Anti-Human IgG/APC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Use assay conditions validated for FCM, apply Goat Anti-Human IgG/APC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Precautions
Store at 2-8°C, handle with care, and optimize antibody dilution for each application to minimize background and ensure specific binding.
Validation Images