Goat anti-Human albumin
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mg | CAB.43027-1mg | €37.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
5mg | CAB.43027-5mg | €2085.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
10mg | CAB.43027-10mg | €2085.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100mg | CAB.43027-100mg | €2085.00 |
| Catalog No | CAB.43027 |
|---|---|
| Product Name | Goat anti-Human albumin |
| Isotype | IgG |
| Calculated MW | 66kDa |
| Immunogen | Human Serum Albumin |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | Polyclonal Antibody |
| Reactivity | Human |
| Application | ELISA WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purification |
| Storage Buffer | Buffer: PBS with 0.03% Proclin300 （PH7.2-7.4). |
| Storage | Shipped at 4℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Albumin is a globular unglycosylated serum protein of molecular weight 65，000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product，proalbumin，is in turn cleaved in the Golgi vesicles to produce the secreted albumin. [provided by RefSeq，Jul 2008].
Protocol / Instructions
The Goat anti-Human albumin antibody is designed for the detection of human albumin in various applications.
For ELISA, the recommended dilution is 1:2000-100000. Reagents and buffers required include a 96-well plate, coating buffer, blocking buffer, and substrate. Sample preparation involves coating the plate with antigen, followed by blocking and washing steps. The primary antibody is then added, and CABter incubation, a secondary antibody conjugated to an enzyme is applied. The reaction is developed using a substrate, and the absorbance is measured. Technical notes: Optimize the coating antigen concentration and antibody dilution for best results.
For Western Blotting, the recommended dilution is 1:1000-20000. Required reagents and buffers include sample buffer, running buffer, transfer buffer, and blocking buffer. Sample preparation involves mixing the sample with sample buffer and heating, followed by SDS-PAGE and transfer to a membrane. The membrane is then blocked, and the primary antibody is added. CABter incubation and washing, a secondary antibody conjugated to an enzyme or fluorescent tag is applied. Detection is achieved using a substrate or fluorescence imaging. Technical notes: Use a suitable positive control to verify the specificity of the antibody.
Use assay conditions validated for ELISA WB, apply Goat anti-Human albumin at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: WB 1:1000-20000.;ELISA:1:2000-100000..
Precautions
Store at -20°C. Handle with care, and avoid repeated freeze-thaw cycles. Optimize antibody dilution and incubation times for best results.
Validation Images