Goat Anti-Guinea Pig IgG/FITC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.43251-100ul | €100.00 |
| Catalog No | CAB.43251 |
|---|---|
| Product Name | Goat Anti-Guinea Pig IgG/FITC |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Guinea Pig IgG |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat Anti-Guinea Pig IgG/FITC is a versatile antibody that can be used in various applications.
For IF, the following reagents are required: Goat Anti-Guinea Pig IgG/FITC, phosphate-buffered saline (PBS), and a fluorescent microscope.
Sample preparation involves fixing and permeabilizing cells or tissue sections.
The antibody should be diluted to 1:100-1000 in PBS and incubated with the sample for 1-2 hours at room temperature.
CABter washing with PBS, the sample can be visualized using a fluorescent microscope.
Technical notes: Optimize the antibody dilution and incubation time for best results.
For FCM, the following reagents are required: Goat Anti-Guinea Pig IgG/FITC, PBS, and a flow cytometer.
Sample preparation involves isolating cells and washing them with PBS.
The antibody should be diluted to 1:100-1000 in PBS and incubated with the cells for 30 minutes to 1 hour on ice.
CABter washing with PBS, the cells can be analyzed using a flow cytometer.
Technical notes: Use a suitable isotype control to verify the specificity of the antibody.
Use assay conditions validated for IF, apply Goat Anti-Guinea Pig IgG/FITC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Use assay conditions validated for FCM, apply Goat Anti-Guinea Pig IgG/FITC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Precautions
Store at 2-8°C. Handle with care. Use appropriate controls and optimize antibody dilution for best results.
Validation Images