Streptavidin/RBITC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
25T | CAB.43301-25T | €105.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100T | CAB.43301-100T | €220.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.43301-100ul | €42.50 |
| Catalog No | CAB.43301 |
|---|---|
| Product Name | Streptavidin/RBITC |
| Isotype | N/A |
| Calculated MW | N/A |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Streptavidin |
| Clone Type | N/A |
| Reactivity | Streptavidin |
| Application | IF;ICC |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Streptavidin/RBITC conjugate is a valuable tool for various applications, including Immunofluorescence (IF) and Immunocytochemistry (ICC).
For IF, start by preparing your samples, either by fixing cells or tissue sections. Use a fixative such as paraformaldehyde, followed by permeabilization with a detergent like Triton X-100.
Next, block non-specific binding sites with a blocking buffer containing bovine serum albumin or normal serum.
Then, incubate the samples with the biotinylated primary antibody of interest, followed by the Streptavidin/RBITC conjugate at a dilution of 1:100 to 1:1000.
CABter incubation, wash the samples thoroughly with a washing buffer to remove excess conjugate.
Finally, visualize the signal using a fluorescence microscope.
Block non-specific binding sites, then incubate with the biotinylated primary antibody.
Subsequently, incubate with the Streptavidin/RBITC conjugate at the recommended dilution.
Wash the cells and visualize the signal.
Technical notes: Optimize the dilution of the Streptavidin/RBITC conjugate for your specific application and use appropriate controls to ensure specificity of the signal.
Use assay conditions validated for IF, apply Streptavidin/RBITC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: ICC=1:100-1000;
IF=1:100-1000.
Use assay conditions validated for ICC, apply Streptavidin/RBITC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: ICC=1:100-1000;
IF=1:100-1000.
Precautions
Store at 2-8°C, handle with care, and optimize conjugate dilution for each application to avoid non-specific binding.
Validation Images