Avidin/HRP
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.1ml | CAB.43348-0.1ml | €55.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1ml | CAB.43348-1ml | €375.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
20ul | CAB.43348-20ul | €100.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.43348-50ul | €500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.43348-100ul | €500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul(PBS Only) | CAB.43348-100ul(PBS Only) | €500.00 |
| Catalog No | CAB.43348 |
|---|---|
| Product Name | Avidin/HRP |
| Isotype | N/A |
| Calculated MW | N/A |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Avidin |
| Clone Type | N/A |
| Reactivity | Avidin |
| Application | IHC-P;IHC-F;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Avidin/HRP antibody is a versatile tool for detecting avidin in various applications. The following protocols are recommended for each validated application.
Reagents or buffers: PBS, Tween-20, non-fat dry milk, and ECL substrate.
Sample preparation: Separate proteins by SDS-PAGE and transfer to a PVDF membrane.
Antibody incubation: Incubate the membrane with Avidin/HRP antibody at a dilution of 1:500-5000 in PBS with 0.1% Tween-20 and 5% non-fat dry milk for 1 hour at room temperature.
Washing or detection: Wash the membrane three times with PBS containing 0.1% Tween-20, and detect using ECL substrate.
Technical notes: Optimize the antibody dilution and incubation time for best results.
Reagents or buffers: PBS, Triton X-100, and DAB substrate.
Sample preparation: Fix and embed tissue samples, then section and mount on slides.
Antibody incubation: Incubate the slides with Avidin/HRP antibody at a dilution of 1:400-800 in PBS with 0.1% Triton X-100 for 1 hour at room temperature.
Washing or detection: Wash the slides three times with PBS, and detect using DAB substrate.
Technical notes: Use a suitable antigen retrieval method if necessary.
Reagents or buffers: PBS, Triton X-100, and fluorescent secondary antibody.
Sample preparation: Fix and permeabilize cells or tissue samples, then incubate with primary antibody.
Antibody incubation: Incubate the samples with Avidin/HRP antibody at a dilution of 1:400-800 in PBS with 0.1% Triton X-100 for 1 hour at room temperature.
Washing or detection: Wash the samples three times with PBS, and detect using a fluorescent secondary antibody.
Technical notes: Optimize the antibody dilution and incubation time for best results.
Reagents or buffers: PBS, Tween-20, and TMB substrate.
Sample preparation: Coat ELISA plates with avidin and block with non-fat dry milk.
Antibody incubation: Incubate the plates with Avidin/HRP antibody at a dilution of 1:500-5000 in PBS with 0.1% Tween-20 for 1 hour at room temperature.
Washing or detection: Wash the plates three times with PBS containing 0.1% Tween-20, and detect using TMB substrate.
Technical notes: Optimize the antibody dilution and incubation time for best results.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation time for best results.
Validation Images