Goat Anti-Mouse IgM antibody
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
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Available – Dispatch in 14 days
Global Network - International delivery
|
1mg | CAB.45199-1mg | €500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
10mg | CAB.45199-10mg | €2500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
20ul | CAB.45199-20ul | €100.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.45199-50ul | €500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.45199-100ul | €500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul(PBS Only) | CAB.45199-100ul(PBS Only) | €500.00 |
| Catalog No | CAB.45199 |
|---|---|
| Product Name | Goat Anti-Mouse IgM antibody |
| Isotype | IgG |
| Calculated MW | 950kDa |
| Immunogen | Native Mouse IgM purified from Mouse plasma |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | Polyclonal Antibody |
| Reactivity | Mouse |
| Application | ELISA WB IHC-P IHC-F |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein G |
| Storage Buffer | 0.01M PBS(pH7.4) |
| Storage | Store at -20℃ stable for 2 years (lyophilized powder). Avoid repeated freeze/thaw cycles |
Background
Immunoglobulin M (IgM) normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences.
Protocol / Instructions
The Goat Anti-Mouse IgM antibody is designed for use in various applications including ELISA, Western Blotting, Immunohistochemistry on paraffin sections (IHC-P), and Immunohistochemistry on frozen sections (IHC-F). The recommended dilution for each application is provided, but optimal dilution may vary depending on the specific experimental conditions.
For ELISA, the recommended dilution is 1:2000-10000. Reagents needed include the Goat Anti-Mouse IgM antibody, antigen of interest, ELISA plates, blocking buffer, and detection reagents. Sample preparation involves coating the ELISA plate with the antigen, followed by blocking to reduce non-specific binding. The Goat Anti-Mouse IgM antibody is then incubated with the antigen-coated plate, followed by washing and detection using an enzyme-linked secondary antibody. Technical notes: Ensure proper plate washing between steps to minimize background.
For Western Blotting, the recommended dilution is 1:1000-5000. Required reagents include the Goat Anti-Mouse IgM antibody, sample lysates, SDS-PAGE gel, transfer membrane, and detection reagents. Sample preparation involves resolving the sample proteins by SDS-PAGE and transferring them to a membrane. The membrane is then blocked to reduce non-specific binding, followed by incubation with the Goat Anti-Mouse IgM antibody. CABter washing, detection is performed using an appropriate secondary antibody. Technical notes: Optimize blocking conditions to reduce background.
For Immunohistochemistry on paraffin sections (IHC-P), the recommended dilution is 1:100-500. Necessary reagents include the Goat Anti-Mouse IgM antibody, paraffin-embedded tissue sections, antigen retrieval solution, and detection reagents. Sample preparation involves deparaffinizing and rehydrating the tissue sections, followed by antigen retrieval to unmask epitopes. The Goat Anti-Mouse IgM antibody is then incubated with the sections, followed by washing and detection using an enzyme-linked secondary antibody. Technical notes: Antigen retrieval conditions may need optimization for specific tissues.
For Immunohistochemistry on frozen sections (IHC-F), the recommended dilution is 1:200-1000. Required reagents include the Goat Anti-Mouse IgM antibody, frozen tissue sections, blocking buffer, and detection reagents. Sample preparation involves fixing and permeabilizing the tissue sections. The Goat Anti-Mouse IgM antibody is then incubated with the sections, followed by washing and detection using an enzyme-linked secondary antibody. Technical notes: Fixation and permeabilization conditions can significantly CABfect antibody binding and should be optimized for each tissue type.
Use assay conditions validated for ELISA WB IHC-P IHC-F, apply Goat Anti-Mouse IgM antibody at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: WB 1:1000-5000.;ELISA 1:2000-10000.;IHC-P 1:100-500.;IHC-F 1:200-1000..
Precautions
Store at 2-8°C. Handle with care to avoid contamination. Use appropriate controls and optimize antibody dilution for each application.
Validation Images