Donkey Anti-Goat IgG/Solar Fluor 488
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.45363-100ul | €87.50 |
| Catalog No | CAB.45363 |
|---|---|
| Product Name | Donkey Anti-Goat IgG/Solar Fluor 488 |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Goat IgG/Solar Fluor 488 is a secondary antibody used for detecting goat IgG primary antibodies.
Reagents or buffers: phosphate-buffered saline (PBS), mounting medium, and a fluorescence microscope.
Sample preparation: fix and permeabilize cells or tissue sections, then block with a blocking buffer.
Antibody incubation: incubate samples with the primary goat antibody, followed by the Donkey Anti-Goat IgG/Solar Fluor 488 secondary antibody at a recommended dilution of 1:50-500.
Washing or detection: wash samples with PBS, then mount and visualize using a fluorescence microscope.
Technical notes: optimize the dilution of the secondary antibody and the incubation time for the best signal-to-noise ratio.
Controls: include a negative control without the primary antibody to assess non-specific binding.
Use assay conditions validated for IF, apply Donkey Anti-Goat IgG/Solar Fluor 488 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:50-500.
Precautions
Store at 2-8°C, handle with care, and optimize antibody dilution for best results.
Validation Images