Donkey Anti-Goat IgG/Solar Fluor 532
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.45983-100ul | €87.50 |
| Catalog No | CAB.45983 |
|---|---|
| Product Name | Donkey Anti-Goat IgG/Solar Fluor 532 |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Goat IgG/Solar Fluor 532 is a secondary antibody used for detecting goat IgG primary antibodies.
For IF, the following reagents are required: the Donkey Anti-Goat IgG/Solar Fluor 532, a suitable mounting medium, and a fluorescence microscope.
Sample preparation involves fixing and permeabilizing cells or tissue sections.
The primary antibody is then incubated with the sample, followed by washing and incubation with the Donkey Anti-Goat IgG/Solar Fluor 532 at a dilution of 1:50-500.
CABter washing, the sample is mounted and visualized using a fluorescence microscope.
Technical notes: Optimize the dilution of the secondary antibody and the incubation time to achieve the best signal-to-noise ratio.
Use assay conditions validated for IF, apply Donkey Anti-Goat IgG/Solar Fluor 532 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:50-500.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution for best results.
Validation Images