Donkey Anti-Human IgG/Cy3
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
20ul | CAB.45986-20ul | €100.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.45986-50ul | €500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.45986-100ul | €150.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul(PBS Only) | CAB.45986-100ul(PBS Only) | €500.00 |
| Catalog No | CAB.45986 |
|---|---|
| Product Name | Donkey Anti-Human IgG/Cy3 |
| Isotype | N/A |
| Calculated MW | 160kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Human IgG |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Human IgG/Cy3 is a versatile antibody suitable for various applications, including Immunofluorescence (IF) and Flow Cytometry (FCM).
Reagents or buffers: Phosphate-buffered saline (PBS), 4% paraformaldehyde, 0.1% Triton X-100, and mounting medium.
Sample preparation: Fix cells or tissue sections with 4% paraformaldehyde, permeabilize with 0.1% Triton X-100, and block non-specific binding sites.
Antibody incubation: Incubate samples with Donkey Anti-Human IgG/Cy3 at a dilution of 1:100-1000 in PBS for 1-2 hours at room temperature or overnight at 4°C.
Washing or detection: Wash samples with PBS and mount with mounting medium.
Technical notes: Optimize antibody dilution and incubation time for specific applications.
Reagents or buffers: PBS, 1% bovine serum albumin (BSA), and 0.1% sodium azide.
Sample preparation: Prepare cell suspensions and block non-specific binding sites with 1% BSA.
Antibody incubation: Incubate cells with Donkey Anti-Human IgG/Cy3 at a dilution of 1:100-1000 in PBS for 30 minutes to 1 hour on ice.
Washing or detection: Wash cells with PBS and analyze by flow cytometry.
Technical notes: Optimize antibody dilution and incubation time for specific cell types and flow cytometers.
Use assay conditions validated for IF, apply Donkey Anti-Human IgG/Cy3 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:100-1000;
FCM=1:100-1000.
Use assay conditions validated for FCM, apply Donkey Anti-Human IgG/Cy3 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:100-1000;
FCM=1:100-1000.
Precautions
Store at 2-8°C, handle with care, and optimize antibody dilution and incubation time for specific applications to minimize non-specific binding and ensure reliable results.
Validation Images