Donkey Anti-Goat IgG/Solar Fluor 350
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.47131-100ul | €87.50 |
| Catalog No | CAB.47131 |
|---|---|
| Product Name | Donkey Anti-Goat IgG/Solar Fluor 350 |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Goat IgG/Solar Fluor 350 is a secondary antibody used for detecting goat IgG primary antibodies.
For IF, the following steps are recommended:
- Reagents or buffers: phosphate-buffered saline (PBS), 4% paraformaldehyde for fixation, and a mounting medium with DAPI for nuclear staining.
- Sample preparation: fix cells or tissue sections with 4% paraformaldehyde, permeabilize with 0.1% Triton X-100 if necessary, and block non-specific binding sites with 5% normal donkey serum.
- Antibody incubation: incubate the sample with the primary goat IgG antibody, followed by incubation with the Donkey Anti-Goat IgG/Solar Fluor 350 secondary antibody at a dilution of 1:50-500.
- Washing or detection: wash the sample thoroughly with PBS CABter each antibody incubation, and detect the signal using a fluorescence microscope.
- Technical notes: optimize the dilution of the secondary antibody and the incubation time to achieve the best signal-to-noise ratio.
## Troubleshooting and Optimization
Optimize the fixation and permeabilization steps to preserve the epitope and maintain tissue or cell morphology. Use controls, such as omitting the primary antibody, to verify the specificity of the staining.
Use assay conditions validated for IF, apply Donkey Anti-Goat IgG/Solar Fluor 350 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:50-500.
Precautions
Store at 2-8°C, handle with care, and optimize antibody dilution and incubation time to minimize non-specific binding.
Validation Images