Donkey Anti-Guinea Pig IgG/Cy7
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.47232-100ul | €200.00 |
| Catalog No | CAB.47232 |
|---|---|
| Product Name | Donkey Anti-Guinea Pig IgG/Cy7 |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Guinea Pig IgG |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Guinea Pig IgG/Cy7 is a highly specific antibody conjugate designed for various applications, including Immunofluorescence (IF).
- Reagents or Buffers: Phosphate-buffered saline (PBS), 4% paraformaldehyde for fixation, 0.1% Triton X-100 for permeabilization, and a blocking buffer (e.g., 5% normal donkey serum in PBS).
- Sample Preparation: Fix and permeabilize cells or tissue sections, then block with the blocking buffer to reduce non-specific binding.
- Antibody Incubation: Dilute the Donkey Anti-Guinea Pig IgG/Cy7 in the blocking buffer at a recommended dilution of 1:100-1000. Incubate the samples with the diluted antibody for 1-2 hours at room temperature or overnight at 4°C.
- Washing or Detection: Wash the samples thoroughly with PBS CABter incubation. Detect the fluorescence using a fluorescence microscope with appropriate filters for Cy7.
## Technical Notes
Optimize the antibody dilution and incubation time based on the specific application and sample type. The use of a mounting medium that reduces photobleaching is recommended for fluorescence preservation.
Use assay conditions validated for IF, apply Donkey Anti-Guinea Pig IgG/Cy7 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:100-1000.
Precautions
Store at 2-8°C. Handle with care, and avoid repeated freeze-thaw cycles. Use appropriate controls and optimize antibody dilution for specific applications.
Validation Images