Goat Anti-Chicken IgG/HRP
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.47823-100ul | €100.00 |
| Catalog No | CAB.47823 |
|---|---|
| Product Name | Goat Anti-Chicken IgG/HRP |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Chicken IgG |
| Application | IHC-F;IHC-P;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein G |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat Anti-Chicken IgG/HRP antibody is a versatile tool for various immunological applications. Below are the protocols for each validated application.
Reagents or buffers: Tris-buffered saline with Tween 20 (TBST), skim milk, and Goat Anti-Chicken IgG/HRP antibody.
Sample preparation: Separate proteins by SDS-PAGE and transfer to a membrane.
Antibody incubation: Incubate the membrane with the Goat Anti-Chicken IgG/HRP antibody at a dilution of 1:1000-10000 in TBST with 5% skim milk for 1 hour at room temperature.
Washing or detection: Wash the membrane three times with TBST, then detect using an appropriate ECL substrate.
Technical notes: Optimize the blocking conditions and antibody dilution to minimize background and enhance signal.
Reagents or buffers: Phosphate-buffered saline (PBS), normal goat serum, and Goat Anti-Chicken IgG/HRP antibody.
Sample preparation: Fix and embed tissue samples, then section and mount on slides.
Antibody incubation: Incubate the slides with the Goat Anti-Chicken IgG/HRP antibody at a dilution of 1:100-1000 in PBS with 1% normal goat serum for 30 minutes at room temperature.
Washing or detection: Wash the slides three times with PBS, then detect using an appropriate chromogen substrate.
Technical notes: Perform antigen retrieval if necessary, and optimize the antibody dilution to achieve the best staining results.
Reagents or buffers: Phosphate-buffered saline (PBS), normal goat serum, and Goat Anti-Chicken IgG/HRP antibody.
Sample preparation: Fix and permeabilize cells or tissue samples, then mount on slides.
Antibody incubation: Incubate the slides with the Goat Anti-Chicken IgG/HRP antibody at a dilution of 1:100-1000 in PBS with 1% normal goat serum for 30 minutes at room temperature.
Washing or detection: Wash the slides three times with PBS, then detect using an appropriate fluorochrome-conjugated secondary antibody.
Technical notes: Optimize the fixation and permeabilization conditions to preserve the antigen and cell structure.
Reagents or buffers: Coating buffer, blocking buffer, and Goat Anti-Chicken IgG/HRP antibody.
Sample preparation: Coat the ELISA plate with the antigen, then block with a blocking buffer.
Antibody incubation: Incubate the plate with the Goat Anti-Chicken IgG/HRP antibody at a dilution of 1:1000-10000 in the blocking buffer for 1 hour at room temperature.
Washing or detection: Wash the plate three times with PBS, then detect using an appropriate chromogen substrate.
Technical notes: Optimize the coating and blocking conditions to minimize non-specific binding and enhance the signal.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution and reaction conditions to achieve the best results.
Validation Images