Donkey Anti-Goat IgG/Cy3
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.48123-100ul | €67.50 |
| Catalog No | CAB.48123 |
|---|---|
| Product Name | Donkey Anti-Goat IgG/Cy3 |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Goat IgG/Cy3 is a secondary antibody used for detecting goat IgG primary antibodies. It is conjugated to the fluorescent dye Cy3, which emits at 570 nm.
- Reagents or buffers: Phosphate-buffered saline (PBS), 4% paraformaldehyde (PFA) for fixation, 0.1% Triton X-100 for permeabilization, and 1% bovine serum albumin (BSA) for blocking.
- Sample preparation: Fix cells or tissue sections with 4% PFA, permeabilize with 0.1% Triton X-100, and block with 1% BSA.
- Antibody incubation: Incubate the sample with the primary goat IgG antibody, followed by the Donkey Anti-Goat IgG/Cy3 secondary antibody at a dilution of 1:50-500.
- Washing or detection: Wash the sample thoroughly with PBS CABter each antibody incubation. Detect the fluorescence signal using a fluorescence microscope.
- Technical notes: Optimize the dilution of the secondary antibody and the incubation time to achieve the best signal-to-noise ratio.
## Technical Considerations
The Donkey Anti-Goat IgG/Cy3 is highly specific for goat IgG and can be used in various IF applications, including co-staining with other antibodies. However, it is essential to optimize the staining protocol for each specific application to achieve optimal results.
Use assay conditions validated for IF, apply Donkey Anti-Goat IgG/Cy3 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:50-500.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation time for best results.
Validation Images