Goat anti-Equine IgG/FITC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.3ml | CAB.48171-0.3ml | €42.50 |
| Catalog No | CAB.48171 |
|---|---|
| Product Name | Goat anti-Equine IgG/FITC |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Equine IgG |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein G |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat anti-Equine IgG/FITC is a fluorescently labeled antibody designed for use in various applications. Below are the protocols for the validated application.
For IF, the following reagents and steps are recommended:
- Reagents: Goat anti-Equine IgG/FITC, mounting medium, and appropriate positive and negative controls.
- Sample Preparation: Prepare samples according to standard protocols for IF. This may include fixation, permeabilization, and blocking.
- Antibody Incubation: Incubate the sample with the Goat anti-Equine IgG/FITC at a dilution of 1:50-500 for 1-2 hours at room temperature or overnight at 4°C.
- Washing or Detection: Wash the sample thoroughly with a suitable buffer CABter incubation. Detect the fluorescence using a fluorescence microscope.
- Technical Notes: Optimize the dilution of the antibody and the incubation time based on the specific application and sample.
## Technical Considerations
The Goat anti-Equine IgG/FITC is specific for Equine IgG and should be used accordingly. Always handle the antibody with care and follow proper protocols for IF to ensure optimal results.
Use assay conditions validated for IF, apply Goat anti-Equine IgG/FITC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:50-500.
Precautions
Store at 2-8°C, handle with care, and optimize antibody dilution and incubation time for best results.
Validation Images