Donkey Anti-Guinea Pig IgG/Cy5
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.48435-50ul | €200.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.48435-100ul | €200.00 |
| Catalog No | CAB.48435 |
|---|---|
| Product Name | Donkey Anti-Guinea Pig IgG/Cy5 |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Guinea Pig IgG |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Guinea Pig IgG/Cy5 is a highly specific antibody conjugate designed for various applications, including Immunofluorescence (IF) and Flow Cytometry (FCM). The recommended dilution for both IF and FCM is 1:100-1000.
Reagents or buffers required include the Donkey Anti-Guinea Pig IgG/Cy5, phosphate-buffered saline (PBS), and a mounting medium with DAPI for nuclear staining. Sample preparation involves fixing cells or tissue sections with a suitable fixative, followed by permeabilization if necessary. The antibody incubation step involves applying the Donkey Anti-Guinea Pig IgG/Cy5 at the recommended dilution in PBS for 1-2 hours at room temperature or overnight at 4°C. Washing is performed with PBS, and detection is achieved through fluorescence microscopy. Technical notes: Use a secondary antibody control to verify specificity.
Reagents or buffers required include the Donkey Anti-Guinea Pig IgG/Cy5, PBS, and a flow cytometry buffer. Sample preparation involves preparing single-cell suspensions and blocking non-specific binding sites. The antibody incubation step involves applying the Donkey Anti-Guinea Pig IgG/Cy5 at the recommended dilution in flow cytometry buffer for 30 minutes to 1 hour on ice. Washing is performed with flow cytometry buffer, and detection is achieved through flow cytometry analysis. Technical notes: Use isotype and secondary antibody controls to verify specificity and rule out non-specific binding.
Use assay conditions validated for IF, apply Donkey Anti-Guinea Pig IgG/Cy5 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Use assay conditions validated for FCM, apply Donkey Anti-Guinea Pig IgG/Cy5 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Precautions
Store at 4°C, handle with care, and optimize antibody dilution for each application to minimize background and ensure specificity.
Validation Images