Anti-5HT1B Polyclonal Antibody
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
20ul | CAB.48573-20ul | €100.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.48573-50ul | €500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.48573-100ul | €500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul(PBS Only) | CAB.48573-100ul(PBS Only) | €500.00 |
| Catalog No | CAB.48573 |
|---|---|
| Product Name | Anti-5HT1B Polyclonal Antibody |
| Isotype | IgG |
| Calculated MW | 44kDa |
| Immunogen | A synthetic peptide of human 5HT1B |
| Public Immunogen Range | 5-55/390aa |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Reactivity | Human(100%);Chimpanzee(100%);Cat(91.7%);Opossums(90%) |
| Application | WB |
| Subcellular Location | Cell membrane |
| Purification Method | CABfinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
Background
The protein encoded by this intronless gene is a G-protein coupled receptor for serotonin (5-hydroxytryptamine). Ligand binding activates second messengers that inhibit the activity of adenylate cyclase and manage the release of serotonin, dopamine, and acetylcholine in the brain. The encoded protein may be involved in several neuropsychiatric disorders and therefore is often a target of antidepressant and other psychotherapeutic drugs.
Protocol / Instructions
The Anti-5HT1B Polyclonal Antibody is a rabbit-derived antibody that targets the 5HT1B protein, a G-protein coupled receptor for serotonin. This antibody has been validated for use in Western Blotting (WB) applications.
Reagents or Buffers: RIPA buffer, protease inhibitors, SDS-PAGE gel, transfer buffer, blocking buffer, and ECL substrate.
Sample Preparation: Harvest cells or tissue samples and lyse in RIPA buffer with protease inhibitors. Separate proteins by SDS-PAGE and transfer to a PVDF membrane.
Antibody Incubation: Incubate the membrane with the Anti-5HT1B Polyclonal Antibody at the recommended dilution in blocking buffer for 1-2 hours at room temperature or overnight at 4°C.
Washing or Detection: Wash the membrane with TBST and detect using an ECL substrate.
Technical Notes: The optimal dilution and incubation time may vary depending on the specific application and sample type. It is recommended to optimize these conditions for each experiment.
Precautions
Store at -20°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation time for each experiment.
Validation Images