Goat anti-BSA (Immune serum)
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.5mL | CAB.48880-0.5mL | €162.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mL | CAB.48880-1mL | €1125.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
5mL | CAB.48880-5mL | €1125.00 |
| Catalog No | CAB.48880 |
|---|---|
| Product Name | Goat anti-BSA (Immune serum) |
| Isotype | N/A |
| Calculated MW | 66kDa |
| Immunogen | BSA |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | Polyclonal Antibody |
| Reactivity | Bovine |
| Application | ELISA;Double Immunodiffusion |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃，Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Bovine Serum Albumin. Albumin is one of the most extensively utilized proteins in the medical industry. Its availability， inherent stability， and ligand binding ability make albumin an essential component in the production of many therapeutic drugs， vaccines and diagnostic tests. Albumin is a soluble， monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids， fatty acids， and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65，000. The human albumin gene is 16，961 nucleotides long from the putative ‘cap‘ site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product， proalbumin， is in turn cleaved in the Golgi vesicles to produce the secreted albumin.This product is reddish transparent liquid， containing 0.03% Proclin300.
Protocol / Instructions
The Goat anti-BSA (Immune serum) is a versatile antibody that can be utilized in various applications, including ELISA and Double Immunodiffusion.
Reagents or buffers: The ELISA protocol requires a coating buffer, such as carbonate-bicarbonate buffer, and a blocking buffer, such as PBS with 1% BSA.
Sample preparation: The sample should be prepared in a suitable buffer, and the antigen should be coated onto the ELISA plate.
Antibody incubation: The Goat anti-BSA (Immune serum) should be diluted to 1:5000-50000 in the blocking buffer, and incubated on the ELISA plate for 1-2 hours at room temperature.
Washing or detection: The plate should be washed with a washing buffer, such as PBS with 0.1% Tween-20, and the signal should be detected using a suitable detection method, such as a chromogenic substrate.
Technical notes: The optimal dilution of the antibody should be determined by titration, and the incubation time and temperature may need to be optimized for specific applications.
Sample preparation: The sample should be prepared in a suitable buffer, and the antigen and antibody should be loaded into the gel.
Antibody incubation: The Goat anti-BSA (Immune serum) should be diluted to 1:4-32 in the buffer, and incubated in the gel for 24-48 hours at room temperature.
Washing or detection: The gel should be washed with a washing buffer, such as PBS, and the precipitin line should be visualized using a suitable staining method, such as Coomassie blue.
Technical notes: The optimal dilution of the antibody should be determined by titration, and the incubation time and temperature may need to be optimized for specific applications.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation conditions to minimize non-specific binding and ensure specific detection of the target antigen.
Validation Images