Goat anti-Rabbit IgG
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mg | CAB.49021-1mg | €25.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
10mg | CAB.49021-10mg | €1562.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100mg | CAB.49021-100mg | €1562.50 |
| Catalog No | CAB.49021 |
|---|---|
| Product Name | Goat anti-Rabbit IgG |
| Isotype | IgG |
| Calculated MW | 150kDa |
| Immunogen | Rabbit IgG full length protein |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | Polyclonal Antibody |
| Reactivity | Rabbit |
| Application | ELISA WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purification |
| Storage Buffer | Buffer: PBS with 0.03% Proclin300（PH7.2-7.4). |
| Storage | Shipped at 4℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Immunoglobulin G (IgG)，is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions;to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Protocol / Instructions
The Goat anti-Rabbit IgG antibody is designed for use in various applications including ELISA and Western Blotting.
For ELISA, the recommended dilution is between 1:5000-100000. Reagents and buffers required include the antigen of interest, coating buffer, blocking buffer, and substrate. Sample preparation involves coating the plate with the antigen, followed by blocking to reduce non-specific binding. The primary antibody, in this case, the antigen of interest, is not used as this is a secondary antibody. The Goat anti-Rabbit IgG is then incubated, allowing it to bind to any rabbit IgG present. Washing steps are crucial to remove unbound antibody, and detection is typically achieved through an enzymatic reaction. Technical notes: Ensure proper plate washing between steps to minimize background noise.
For Western Blotting, the recommended dilution is between 1:1000-20000. Required reagents and buffers include sample buffer, running buffer, transfer buffer, and blocking buffer. Sample preparation involves lysing cells or tissues in sample buffer, followed by separation via SDS-PAGE. The separated proteins are then transferred onto a membrane. The membrane is blocked to reduce non-specific binding, and the Goat anti-Rabbit IgG antibody is incubated, allowing it to bind to any rabbit IgG primary antibody used. Washing steps are necessary to remove unbound antibody, and detection can be achieved through various methods including chemiluminescence. Technical notes: Optimize blocking conditions and antibody dilutions to achieve the best signal-to-noise ratio.
Use assay conditions validated for ELISA WB, apply Goat anti-Rabbit IgG at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: WB 1:1000-20000.;ELISA:1:5000-100000..
Precautions
Store at -20°C, handle with care, include appropriate controls, and optimize antibody dilutions for best results.
Validation Images