Goat Anti-Human Albumin/HRP
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.49037-100ul | €35.00 |
| Catalog No | CAB.49037 |
|---|---|
| Product Name | Goat Anti-Human Albumin/HRP |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Human Albumin |
| Application | IHC-F;IHC-P;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein G |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat Anti-Human Albumin/HRP antibody is a versatile tool for detecting human albumin in various applications.
For Western blotting, prepare samples by lysing cells or tissues in an appropriate buffer, then separate proteins by SDS-PAGE and transfer to a membrane. Block the membrane with 5% non-fat milk in TBS-T, then incubate with the Goat Anti-Human Albumin/HRP antibody at a dilution of 1:1000-10000 in TBS-T. Wash the membrane with TBS-T and detect using a chemiluminescent substrate.
For immunohistochemistry on paraffin-embedded tissues, deparaffinize and rehydrate sections, then perform antigen retrieval using a suitable method. Block endogenous peroxidase and incubate with the Goat Anti-Human Albumin/HRP antibody at a dilution of 1:100-1000. Use a suitable detection system and counterstain with hematoxylin.
For immunohistochemistry on frozen tissues, fix sections in acetone, then incubate with the Goat Anti-Human Albumin/HRP antibody at a dilution of 1:100-1000. Use a suitable detection system and counterstain with DAPI or another nuclear stain.
For ELISA, coat plates with human albumin antigen, then block with 5% non-fat milk in PBS. Incubate with the Goat Anti-Human Albumin/HRP antibody at a dilution of 1:1000-10000, followed by a suitable substrate for detection.
Technical notes: Optimize antibody dilutions and incubation times for each specific application. Use appropriate controls to verify specificity and sensitivity.
Precautions
Store at 2-8°C. Handle with care, avoid repeated freeze-thaw cycles. Use appropriate controls to verify specificity and optimize assay conditions.
Validation Images