Mouse Anti-Human IgG(3D3)/PE
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
20T | CAB.50021-20T | €157.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
20ul | CAB.50021-20ul | €100.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.50021-50ul | €240.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100T | CAB.50021-100T | €397.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.50021-100ul | €200.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul(PBS Only) | CAB.50021-100ul(PBS Only) | €500.00 |
| Catalog No | CAB.50021 |
|---|---|
| Product Name | Mouse Anti-Human IgG(3D3)/PE |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Mouse |
| Clone Type | N/A |
| Reactivity | Human IgG |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Mouse Anti-Human IgG(3D3)/PE antibody is a valuable tool for various immunological applications. Below are detailed protocols for its validated applications.
For IF, the following steps are recommended:
- Reagents or buffers: phosphate-buffered saline (PBS), 4% paraformaldehyde for fixation, 0.1% Triton X-100 for permeabilization.
- Sample preparation: Fix cells with 4% paraformaldehyde, permeabilize with 0.1% Triton X-100.
- Antibody incubation: Incubate with Mouse Anti-Human IgG(3D3)/PE at a dilution of 1:100-1000 in PBS for 1 hour at room temperature.
- Washing or detection: Wash with PBS three times, then proceed with fluorescence microscopy.
- Technical notes: Optimize the dilution based on the specific experimental conditions and the intensity of the fluorescence signal.
For FCM, follow these guidelines:
- Reagents or buffers: PBS, 1% bovine serum albumin (BSA) for blocking.
- Sample preparation: Prepare cell suspensions and block with 1% BSA in PBS.
- Antibody incubation: Incubate cells with Mouse Anti-Human IgG(3D3)/PE at a dilution of 1:100-1000 in PBS for 30 minutes on ice.
- Washing or detection: Wash cells with PBS, then analyze by flow cytometry.
- Technical notes: Ensure proper compensation for the PE channel, and consider using isotype controls for specificity.
Both IF and FCM protocols require optimization of the antibody dilution to achieve the best results. It is crucial to consider the specific experimental conditions and the characteristics of the samples being analyzed.
Use assay conditions validated for IF, apply Mouse Anti-Human IgG(3D3)/PE at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution for each application to ensure specificity and sensitivity.
Validation Images