Goat anti-Equine IgG/Gold 15nm
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.50066-50ul | €595.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.50066-100ul | €960.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
500ul | CAB.50066-500ul | €42.50 |
| Catalog No | CAB.50066 |
|---|---|
| Product Name | Goat anti-Equine IgG/Gold 15nm |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Equine IgG |
| Application | GICA;IGS;IEM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein G |
| Storage Buffer | N/A |
| Storage | Store at 2-8℃ for 3-6 months. Avoid repeated freeze/thaw cycles. |
Background
The particle size is 15nm
Protocol / Instructions
The Goat anti-Equine IgG/Gold 15nm antibody is designed for use in various immunocytochemical and immunogold labeling techniques. The recommended dilution ranges for different applications are provided, but optimal dilutions may vary depending on the specific experimental conditions.
For Gel Immunogold Chromatography Assay (GICA), start by preparing the sample and column according to the manufacturer's instructions. Use a dilution of 1:20-200 of the Goat anti-Equine IgG/Gold 15nm antibody in the recommended buffer. Apply the sample to the column and incubate for the recommended time. Wash the column with the washing buffer, and then elute the bound fraction. The eluted fraction can be analyzed for the presence of Equine IgG using techniques such as spectrophotometry or immunoblotting.
For Immunogold Staining (IGS), prepare the sample by fixing and sectioning according to standard protocols. Incubate the sections with a dilution of 1:20-200 of the Goat anti-Equine IgG/Gold 15nm antibody in the recommended buffer for 1-2 hours at room temperature. Wash the sections with the washing buffer, and then incubate with the secondary antibody if required. Detect the signal using a transmission electron microscope.
For Immunoelectron Microscopy (IEM), prepare the sample by fixing and sectioning according to standard protocols. Incubate the sections with a dilution of 1:20-200 of the Goat anti-Equine IgG/Gold 15nm antibody in the recommended buffer for 1-2 hours at room temperature. Wash the sections with the washing buffer, and then incubate with the secondary antibody if required. Detect the signal using a transmission electron microscope. Technical notes: The choice of buffer and washing conditions may CABfect the outcome of the experiment. Optimize the conditions for the specific application to achieve the best results.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution for best results.
Validation Images