Goat anti-Equine IgG (Immune serum)
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.5mL | CAB.51471-0.5mL | €162.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mL | CAB.51471-1mL | €1125.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
5mL | CAB.51471-5mL | €1125.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.51471-50ul | €272.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.51471-100ul | €490.00 |
| Catalog No | CAB.51471 |
|---|---|
| Product Name | Goat anti-Equine IgG (Immune serum) |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | Equine IgG full length protein |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | Polyclonal Antibody |
| Reactivity | Equine |
| Application | ELISA;WB;Double Immunodiffusion |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃，Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Immunoglobulin G (IgG)， is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. This product is reddish transparent liquid， containing 0.03% Proclin300.
Protocol / Instructions
This antibody is a Goat anti-Equine IgG (Immune serum) that can be used in various applications such as ELISA, Western Blotting, and Double Immunodiffusion.
For ELISA, the recommended dilution is 1:5000-700000. The reagents required include the antigen of interest, a 96-well plate, and a detection system. Sample preparation involves coating the 96-well plate with the antigen and blocking with a blocking buffer. The antibody incubation step involves adding the diluted antibody to the wells and incubating for 1-2 hours at room temperature. Washing with a washing buffer is necessary before detection. Technical notes: Optimize the antibody dilution and incubation time for best results.
For Western Blotting, the recommended dilution is 1:1000-20000. The reagents required include a transfer membrane, a blocking buffer, and a detection system. Sample preparation involves separating the proteins by SDS-PAGE and transferring them to a membrane. The antibody incubation step involves adding the diluted antibody to the membrane and incubating for 1-2 hours at room temperature. Washing with a washing buffer is necessary before detection. Technical notes: Use a suitable blocking buffer to reduce non-specific binding.
For Double Immunodiffusion, the recommended dilution is 1:8-64. The reagents required include an agar plate, a trough, and a detection system. Sample preparation involves preparing the antigen and antibody solutions. The antibody incubation step involves adding the diluted antibody to the trough and allowing it to diffuse towards the antigen. Washing is not required, but the plate should be incubated at room temperature for several hours to allow diffusion. Technical notes: Optimize the antibody dilution and incubation time for best results.
Precautions
Store at -20°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation time for best results.
Validation Images