Goat anti-Human IgG-Fc (Immune serum)
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.5mL | CAB.52138-0.5mL | €387.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mL | CAB.52138-1mL | €2750.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
5mL | CAB.52138-5mL | €2750.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
25ug | CAB.52138-25ug | €187.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ug | CAB.52138-100ug | €387.50 |
| Catalog No | CAB.52138 |
|---|---|
| Product Name | Goat anti-Human IgG-Fc (Immune serum) |
| Isotype | N/A |
| Calculated MW | 52kDa |
| Immunogen | Human IgG-Fc purified from human plasma |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | Polyclonal Antibody |
| Reactivity | Human |
| Application | ELISA;WB;Double Immunodiffusion |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃，Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
The Fc region is the constant region on an immunoglobulin molecule， the area that is exactly the same on all antibodies. The Fc region is found on the heavy chains and is not involved in binding antigens.This antibody can significantly recognize IgG FC， but there may be some overlap with IgG Fab， so please choose carefully.This product is reddish transparent liquid， containing 0.03% Proclin300.
Protocol / Instructions
The Goat anti-Human IgG-Fc antibody is designed to recognize the Fc region of human IgG. This antibody can be used in various applications, including ELISA, Western Blotting, and Double Immunodiffusion.
Reagents or buffers: Transfer buffer, blocking buffer, and washing buffer.
Sample preparation: Prepare protein samples and separate them by SDS-PAGE.
Antibody incubation: Incubate the membrane with the Goat anti-Human IgG-Fc antibody at a dilution of 1:2000-20000.
Washing or detection: Wash the membrane with washing buffer and detect using a suitable detection method.
Technical notes: Optimize the antibody dilution and incubation time for best results.
Reagents or buffers: Coating buffer, blocking buffer, and washing buffer.
Sample preparation: Prepare antigen and coat onto ELISA plates.
Antibody incubation: Incubate the plates with the Goat anti-Human IgG-Fc antibody at a dilution of 1:2000-50000.
Washing or detection: Wash the plates with washing buffer and detect using a suitable detection method.
Technical notes: Optimize the antibody dilution and incubation time for best results.
Reagents or buffers: Agar or agarose gel.
Sample preparation: Prepare antigen and antibody samples.
Antibody incubation: Place the samples in the gel and incubate at a dilution of 1:4-32.
Washing or detection: Observe the precipitation lines.
Technical notes: Optimize the antibody dilution and incubation time for best results.
The Goat anti-Human IgG-Fc antibody can significantly recognize IgG Fc, but there may be some overlap with IgG Fab, so please choose carefully.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation time for best results.
Validation Images