Anti-ATP1A1 Recombinant Antibody
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.52650-50ul | €272.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.52650-100ul | €490.00 |
| Catalog No | CAB.52650 |
|---|---|
| Product Name | Anti-ATP1A1 Recombinant Antibody |
| Isotype | IgG |
| Calculated MW | N/A |
| Immunogen | A synthetic peptide of human ATP1A1 |
| Public Immunogen Range | N/A |
| Host | Rabbit |
| Clone Type | Recombinant Antibody |
| Reactivity | Human;Mouse;Rat |
| Application | WB |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | PBS (pH 7.4) containing 50% glycerol，and 0.02% sodium azide. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
Background
The protein encoded by this gene belongs to the family of P-type cation transport ATPases，and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation，for sodium-coupled transport of a variety of organic and inorganic molecules，and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits，a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq，May 2009]
Protocol / Instructions
Reagents or buffers: RIPA buffer, SDS-PAGE gel, transfer buffer, blocking buffer, and ECL substrate.
Sample preparation: Prepare cell lysates using RIPA buffer and separate proteins by SDS-PAGE.
Antibody incubation: Incubate the membrane with Anti-ATP1A1 Recombinant Antibody at a suitable dilution in blocking buffer.
Washing or detection: Wash the membrane with PBST and detect using ECL substrate.
Technical notes: Optimize the dilution of the antibody for best results.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution for best results.
Validation Images