Biotin/HRP
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.1ml | CAB.52844-0.1ml | €37.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1ml | CAB.52844-1ml | €250.00 |
| Catalog No | CAB.52844 |
|---|---|
| Product Name | Biotin/HRP |
| Isotype | N/A |
| Calculated MW | N/A |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Biotin |
| Clone Type | N/A |
| Reactivity | Biotin |
| Application | IHC-P;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Biotin/HRP antibody is a versatile tool for detecting biotinylated molecules in various applications. Below are the protocols for the validated applications.
Reagents or buffers: PBS, Tween-20, non-fat dry milk, biotinylated molecular weight markers.
Sample preparation: Separate proteins by SDS-PAGE and transfer to a PVDF membrane.
Antibody incubation: Incubate the membrane with the Biotin/HRP antibody at a dilution of 1:1000-10000 in PBS with 0.1% Tween-20 and 5% non-fat dry milk for 1 hour at room temperature.
Washing or detection: Wash the membrane three times with PBS containing 0.1% Tween-20, and detect using a chemiluminescent substrate.
Technical notes: Use biotinylated molecular weight markers as positive controls.
Reagents or buffers: PBS, Triton X-100, normal serum, DAB substrate.
Sample preparation: Fix and embed tissue samples, and cut into 4-6 micron sections.
Antibody incubation: Incubate the sections with the Biotin/HRP antibody at a dilution of 1:100-1000 in PBS with 0.1% Triton X-100 and 1% normal serum for 1 hour at room temperature.
Washing or detection: Wash the sections three times with PBS, and detect using a DAB substrate.
Technical notes: Use biotinylated antibodies as positive controls.
Reagents or buffers: PBS, Tween-20, non-fat dry milk, TMB substrate.
Sample preparation: Coat ELISA plates with biotinylated antigens.
Antibody incubation: Incubate the plates with the Biotin/HRP antibody at a dilution of 1:1000-10000 in PBS with 0.1% Tween-20 and 5% non-fat dry milk for 1 hour at room temperature.
Washing or detection: Wash the plates three times with PBS containing 0.1% Tween-20, and detect using a TMB substrate.
Technical notes: Use biotinylated antibodies as positive controls.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilutions for each application.
Validation Images