Donkey Anti-Rabbit IgG/FITC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.53362-100ul | €27.50 |
| Catalog No | CAB.53362 |
|---|---|
| Product Name | Donkey Anti-Rabbit IgG/FITC |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | N/A |
| Clone Type | N/A |
| Reactivity | Rabbit IgG |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Rabbit IgG/FITC is a fluorescently labeled secondary antibody used for detecting rabbit IgG primary antibodies in various applications.
Reagents or buffers: phosphate-buffered saline (PBS), 4% paraformaldehyde, 0.1% Triton X-100, Donkey Anti-Rabbit IgG/FITC.
Sample preparation: Fix cells or tissue sections with 4% paraformaldehyde, permeabilize with 0.1% Triton X-100.
Antibody incubation: Incubate samples with primary rabbit antibody, followed by Donkey Anti-Rabbit IgG/FITC at 1:50-500 dilution.
Washing or detection: Wash samples with PBS, detect fluorescence using a fluorescence microscope.
Technical notes: Optimize antibody dilution and incubation time for best results.
Reagents or buffers: phosphate-buffered saline (PBS), 1% bovine serum albumin (BSA), Donkey Anti-Rabbit IgG/FITC.
Sample preparation: Prepare cell suspensions, block non-specific binding with 1% BSA.
Antibody incubation: Incubate cells with primary rabbit antibody, followed by Donkey Anti-Rabbit IgG/FITC at 1:50-500 dilution.
Washing or detection: Wash cells with PBS, detect fluorescence using a flow cytometer.
Technical notes: Optimize antibody dilution, incubation time, and flow cytometer settings for best results.
Use assay conditions validated for IF, apply Donkey Anti-Rabbit IgG/FITC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:50-500;
IF=1:50-500.
Use assay conditions validated for FCM, apply Donkey Anti-Rabbit IgG/FITC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:50-500;
IF=1:50-500.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation time to minimize non-specific binding.
Validation Images