Goat anti-Chicken IgG/IgY
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mg | CAB.53552-1mg | €62.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
10mg | CAB.53552-10mg | €3750.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100mg | CAB.53552-100mg | €3750.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
25T | CAB.53552-25T | €292.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100T | CAB.53552-100T | €710.00 |
| Catalog No | CAB.53552 |
|---|---|
| Product Name | Goat anti-Chicken IgG/IgY |
| Isotype | IgG |
| Calculated MW | 180kDa |
| Immunogen | Chicken IgY full length protein |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | Polyclonal Antibody |
| Reactivity | Chicken |
| Application | ELISA WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purification |
| Storage Buffer | Buffer: PBS with 0.03% Proclin300 （PH7.2-7.4). |
| Storage | Shipped at 4℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
In chickens，immunoglobulin Y is the functional equivalent to Immunoglobulin G (IgG). Like IgG，it is composed of two light and two heavy chains. Structurally，these two types of immunoglobulin differ primarily in the heavy chains，which in IgY have a molecular mass of about 65，100 atomic mass units (amu)，and are thus larger than in IgG. The light chains in IgY，with a molar mass of about 18，700 amu，are somewhat smaller than the light chains in IgG. The molar mass of IgY thus amounts to about 167，000 amu. The steric flexibility of the IgY molecule is less than that of IgG.Functionally，IgY is partially comparable to Immunoglobulin E (IgE)，as well as to IgG. However，in contrast to IgG，IgY does not bind to Protein A，to Protein G，or to cellular Fc receptors. Furthermore，IgY does not activate the complement system. The name Immunoglobulin Y was suggested in 1969 by G.A. Leslie and L.W. Clem，CABter they were able to show differences between the immunoglobulins found in chicken eggs，and immunoglobulin G. Other synonymous names are Chicken IgG，Egg Yolk IgG，and 7S-IgG.
Protocol / Instructions
The Goat anti-Chicken IgG/IgY antibody is a versatile tool for detecting Chicken IgG/IgY in various applications.
For ELISA, the recommended dilution is 1:5000-100000. Reagents and buffers required include the antigen of interest, coating buffer, blocking buffer, and substrate. Sample preparation involves coating the antigen onto the plate, followed by blocking and washing steps. The antibody is then incubated with the antigen, and CABter washing, the signal is detected using a suitable substrate. Technical notes: Optimize the coating and blocking conditions to minimize non-specific binding.
For Western Blotting, the recommended dilution is 1:1000-20000. Reagents and buffers required include sample buffer, running buffer, transfer buffer, and blocking buffer. Sample preparation involves lysing the cells or tissue, followed by separation using SDS-PAGE and transfer onto a membrane. The membrane is then blocked, and the antibody is incubated with the membrane. CABter washing, the signal is detected using a suitable detection method. Technical notes: Optimize the blocking and washing conditions to minimize non-specific binding.
Use assay conditions validated for ELISA WB, apply Goat anti-Chicken IgG/IgY at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: WB 1:1000-20000.;ELISA:1:5000-100000..
Precautions
Store at -20°C, handle with care, use appropriate controls, and optimize conditions for each application to ensure specificity and sensitivity.
Validation Images