Anti-AAMP Polyclonal Antibody
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
20ul | CAB.55888-20ul | €100.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.55888-50ul | €500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.55888-100ul | €500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul(PBS Only) | CAB.55888-100ul(PBS Only) | €500.00 |
| Catalog No | CAB.55888 |
|---|---|
| Product Name | Anti-AAMP Polyclonal Antibody |
| Isotype | IgG |
| Calculated MW | 47kDa |
| Immunogen | Recombinant protein of human AAMP |
| Public Immunogen Range | 47-397aa |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Reactivity | Cow(98.6%) |
| Application | IHC-P |
| Subcellular Location | Cytoplasm |
| Purification Method | CABfinity purification |
| Storage Buffer | PBS with 50% Glycerol， 0.03% Proclin300， 0.5% BSA， pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
Background
The gene is a member of the immunoglobulin superfamily. The encoded protein is associated with angiogenesis， with potential roles in endothelial tube formation and the migration of endothelial cells. It may also regulate smooth muscle cell migration via the RhoA pathway. The encoded protein can bind to heparin and may mediate heparin-sensitive cell adhesion.
Protocol / Instructions
The Anti-AAMP Polyclonal Antibody is a rabbit-derived antibody directed against the human AAMP protein. This antibody has been validated for use in Immunohistochemistry-Paraffin sections (IHC-P).
Reagents or buffers required include the Anti-AAMP Polyclonal Antibody, paraffin-embedded tissue sections, a detection system such as DAB or alkaline phosphatase, and appropriate positive and negative controls.
Sample preparation involves deparaffinization and rehydration of tissue sections, followed by antigen retrieval using heat-mediated methods in a suitable buffer.
Antibody incubation is performed using the Anti-AAMP Polyclonal Antibody at a recommended dilution of 1:50-200, typically at room temperature for 1-2 hours or at 4°C overnight.
Washing is done with a suitable buffer, such as PBS or TBS, to remove unbound antibody.
Detection is achieved using a secondary antibody conjugated to a detection system, and technical notes include optimization of the primary antibody dilution and incubation time for best results.
Technical notes: The optimal dilution and incubation time may vary depending on the specific application and tissue type. It is recommended to perform a titration experiment to determine the optimal dilution for the specific use case.
Precautions
Store at -20°C, handle with gloves, use appropriate controls, and optimize antibody dilution for best results.
Validation Images